E选择素A561C基因多态性与老年人心肌梗死和血脂的相关分析

Association of A561C polymorphism of the E-selectin with myocardial infarction and serum lipid levels in Chinese elderly

目的 探讨中国湖北武汉地区E选择素基因A5 6 1C多态性与老年人心肌梗死的相关性 ,并对其与血脂、脂蛋白水平的关系进行分析。 方法 应用聚合酶链反应和限制性片段长度多态性 (PCR RFLP)技术检测 198例老年心肌梗死患者、187例中青年心肌梗死患者和 190例老年健康对照者、185例中青年健康对照者E 选择素基因型 ,并按常规方法测定血脂水平。 结果 E 选择素等位基因A、C频率在老年心肌梗死组和中青年心肌梗死组分别为 :92 9%、7 1%和 93 3%、6 7% ;在老年健康对照组和中青年健康对照组分别为 96 6 %、3 4 %和 96 8%、3 2 % ,同龄梗死组与非梗死组间比较 ,差异有显著性 (P <0 0 5 )。基因型频率分布符合Hardy weinberg平衡定律。老年、中青年心肌梗死组分别与老年、中青年健康对照组比较 ,其基因型频率差异有显著性 (均P <0 0 5 ) ;老年、中青年心肌梗死组血浆总胆固醇 (TC)、甘油三酯 (TG)和低密度脂蛋白胆固醇 (LDL C)均高于老年、中青年健康对照组 (P <0 0 5 )。 结论 E 选择素A5 6 1C基因多态性与老年心肌梗死的发病有关 ,C等位基因可能是心肌梗死发病的危险因素之一。

Objective To study the relation between polymorphism of E selectin gene A561C and myocardial infarction (MI) in Wuhan area, China, and to analyse its relation with plasma lipoprotein. Methods Using polymerase chain reaction restriction fragment length polymorphism (PCR RFLP), E selectin A561C genotype was performed in 198 elderly and 187 middle aged patients with myocardial, and 190 elderly and 185 middle aged controls. Other plasma lipid levels were measured by routine methods. Results E selectin allele frequencies of A, C were 92 9%,7 1% and 93 3%,6 7% in 198 elderly patients and 187 young patients respectively; 96 6%, 3 4% in elderly control group and 96 8%, 3 2% in middle aged control group respectively. Genotype distribution was in accordance with Hardy Weinberg equilibrium. The distribution of genotype in E selectin A561C was of significant difference between myocardial infarction group and control group( P <0 05). Both myocardial infarction groups showed higher plasma TC, TG and LDL C than controls( P <0 05). Conclusions E selectin A561C polymorphism is associated with myocardial infarction and C allele may be a risk factor for myocardial infarction in Chinese elderlys.