性病患者中输血传播病毒的检测及其传播途径

Detection and Route of Transmission of Transfusion Transmitted Virus in STD Patients

目的探讨输血传播病毒(transfusiontransmittedvirus,TTV)在性病(STD)患者中的感染状况及经性传播的可能性。方法调查性病患者的性行为相关因素及应用巢式聚合酶链反应(nested-PCR)检测237例STD患者和80例健康体检正常人的血清TTVDNA。结果①STD患者和正常人的血清TTVDNA阳性率分别为19.41%(46/237)和2.5%(2/80),前者TTVDNA的阳性率明显高于后者(P<0.005)。②STD患者男性与女性TTVDNA的阳性率分别为20.59%和17.82%,两者之间差异无显著性(P>0.05)。③不同STD病种患者中以梅毒、生殖器疱疹以及梅毒合并非淋球菌性尿道炎(NGU)和尖锐湿疣(CA)、生殖器疱疹合并NGU患者的TTVDNA的阳性率较高,分别为29.41%、30.43%、33.33%、42.86%、33.33%,而NGU患者的TTVDNA阳性率(10.87%)较低。④28对(56例)STD患者配偶TTVDNA阳性率为28.57%(16/56),11对(22例)正常人配偶TTVDNA的阳性率为4.55%(1/22),两者之间差异有显著性(P<0.05)。⑤TTVDNA阳性组和阴性组的STD患者嫖娼大于半年以上者的人数分别为21例和9例(P<0.005),性伴平均人数分别为17.12和7.86(P<0.001)。结论STD患者中存在严重的TTV感染;嫖娼的时间越长,性伴数越多,感染TTV可能性越大,提示性传播可能是TTV感染途径之一。

Objective To study the situation of infection with transfusion transmitted virus (TTV) and its transmission through sexual contacts in STD patients. Methods Serum TTV DNA was tested by nested-PCR in 237 STD patients and 80 healthy married controls, and the sexual behaviors were investigated among the STD patients. Results (1) The positive rate of TTV DNA in the 237 STD patients was 19.41% (46/237), and it was significantly higher than that in the controls (2.5%, 2/80,P < 0.005). (2) There was no significant difference in positive rates of TTV DNA between male STD patients (20.59%) and female patients (17.82%) (P > 0.05). (3) The positive rates were relatively high in patients with syphilis (29.41%), genital herpes (30.43%), co-infection of syphilis and nongococcal urethritis (NGU) (33.33%), syphilis and condyloma acuminatum (CA) (42.86%) and genital herpes and CA (33.33%), whereas the positive rate of patients with NGU was as low as 10.87%. (4) There was a significant difference in TTV DNA positive rate between 28 STD couples (28.57%, 16/56) and 11 healthy couples (4.55%, 1/22) (P < 0.05). (5) Out of the STD patients with positive TTV DNA, 21 patients had a history to visit sex workers for more than 6 months, whereas there were only 7 patients with such history in the STD patients with negative TTV DNA (P < 0.005). The mean number of sexual partners was 17.20 in STD group with positive TTV DNA, and 7.86 in negative TTV DNA STD group (P < 0.001). Conclusions There is a relatively high TTV infection in the STD patients. The STD patients with a longer history to visit sex workers or more sexual partners are likely to have TTV infection, suggesting that sexual contact is one of transmission routes of TTV.