系统性红斑狼疮FcγRⅡb和C1q抗体的表达及其意义

The expression of FcγRⅡb and anti-C1q antibodies in systemic lupus erythematosus

目的探讨系统性红斑狼疮(SLE)患者外周血单个核细胞FcγRⅡb和血清C1q抗体的表达及其在SLE发病机制中的意义。方法32例SLE和22例正常人外周血粒细胞、淋巴细胞和单核细胞FcγRⅡb的表达采用流式细胞仪测定,血清C1q抗体采用ELISA法测定,并将FcγRⅡb和C1q抗体的表达分别与抗核抗体(ANA)、抗dsDNA抗体及SLEDAI评分作相关性分析。结果SLE患者外周血粒细胞、淋巴细胞和单核细胞FcγRⅡb的表达均减少,以粒细胞和单核细胞为著,血清C1q抗体水平明显增高,且FcγRⅡb和C1q抗体与ANA、抗dsDNA及系统性红斑狼疮活动指数(SLEDAI)评分分别呈负相关和正相关;FcγRⅡb与C1q抗体呈低—中度负相关。结论SLE患者外周血单个核细胞FcγRⅡb表达缺陷和血清C1q抗体水平升高,使免疫复合物的清除功能下降,这在SLE的免疫发病机制中起重要作用,FcγRⅡb和C1q抗体是判断病情活动性的重要指标。

Objective To investigate the expression of FcγRⅡb on peripheral blood mononuclear cells(PBMCs)and serum anti-C1q antibodies in SLE patients and their role in SLE pathogenesis.Methods The ex-pression of FcγRⅡb on peripheral neutrophiles,lymphocytes and monocytes was detected by flowcytometry and the level of anti-C1q antibodies was tested by ELISA in32SLE patients and22healthy individials.At the same time,the correlation between FcγRⅡb,anti-C1q antibodies and ANA,anti-dsDNA antibodies,SLEDAI was eval-uated respectively.Results The expression of FcγRⅡb on peripheral neutrophiles,lymphocytes and monocytes(especially on neutrophiles and monocytes)of SLE patients decreased,the level of anti-C1q antibodies was signifi-cantly increased,compared with that of the control group.FcγRⅡb was negatively and anti-C1q antibodies were positively associated with ANA,anti-dsDNA antibodies and SLEDAI respectively.Conclusion The defective ex-pression of FcγRⅡb on PBMCs and high level of anti-C1q antibodies do exist in SLE patients.FcγRⅡb and anti-C1q antibodies may decrease the clearance of immune complex and play an important role in the pathogene-sis of SLE.They may also be important parameters in indicating SLE activity.