年龄、性别在幼鼠胸腺T细胞增殖中的作用

Effects of sex and age on T cells immune changes in young mice

目的 :分析年幼小鼠性别、年龄与CD3、CD4、CD8免疫参数表达的关系。方法 :分离 3～ 9周小鼠胸腺、脾脏T淋巴细胞 ,FACS分析细胞表面CD3、CD4、CD8的表达 ;CFSE标记后细胞 ,加入ConA、抗TCR抗体、PMA +IONO刺激剂培养 72小时 ,流式细胞仪分析的方法比较不同刺激剂作用下雌、雄小鼠T细胞的增殖。结果 :结果胸腺和脾脏表面CD3、CD4、CD8的表达与性别无明显关系 ,6～ 9周小鼠脾脏CD3+细胞明显多于 3～ 4周小鼠 ;胸腺细胞的增殖与年龄、性别无关 ,而 3周雌性小鼠脾细胞对PMA +IONO刺激后的增殖应答比雄性明显 ,4～ 6周雄性小鼠脾细胞的增殖能力强于雌性小鼠 ,7～ 8周雌性小鼠脾细胞对抗TCR抗体的应答能力明显减小。结论

Objective:Analysis the change in immune parameters of CD4 、CD8、CD3 related to age and sex in young mice. Methods:Spleen and thymus T cells were separated and FACS assayed the expression of CD3 、CD8、CD4 from mice 3 to 9 weeks of age;Proliferation of splenocytes and thymocytes was measured by CFSE in response to in vitro T-cell receptor-dependent mitogens(ConA and anti-TCR),and to a cell surface-receptor-independent mitogenic combination(PMA+IONO) after cultured 72 h. Results:With regard to distribution of immune cells, no significant sex-related changes were seen in thymocyte expression of CD3 、CD8、CD4 or splenocyte expression of CD3 、CD8、CD4.For splenocytes, significantly more cells were positive for CD3 in 6-9 week old compared with 3-4 week old mice. Thymocyte proliferation was not related to age or sex of the mice. For splenocytes of the 3 weeks old mice, the response to a cell of PMA and ionomycin induced a significantly greater than that by cells from females. For mice 7-8 weeks of age, splenocytes from female mice responded significantly less to stimulation by antibody to TCR.Conclusion:Sexual dimorphism in the immune system may be demonstrated prior to puberty.