期刊文献+

年龄、性别在幼鼠胸腺T细胞增殖中的作用 被引量:3

Effects of sex and age on T cells immune changes in young mice
下载PDF
导出
摘要 目的 :分析年幼小鼠性别、年龄与CD3、CD4、CD8免疫参数表达的关系。方法 :分离 3~ 9周小鼠胸腺、脾脏T淋巴细胞 ,FACS分析细胞表面CD3、CD4、CD8的表达 ;CFSE标记后细胞 ,加入ConA、抗TCR抗体、PMA +IONO刺激剂培养 72小时 ,流式细胞仪分析的方法比较不同刺激剂作用下雌、雄小鼠T细胞的增殖。结果 :结果胸腺和脾脏表面CD3、CD4、CD8的表达与性别无明显关系 ,6~ 9周小鼠脾脏CD3+细胞明显多于 3~ 4周小鼠 ;胸腺细胞的增殖与年龄、性别无关 ,而 3周雌性小鼠脾细胞对PMA +IONO刺激后的增殖应答比雄性明显 ,4~ 6周雄性小鼠脾细胞的增殖能力强于雌性小鼠 ,7~ 8周雌性小鼠脾细胞对抗TCR抗体的应答能力明显减小。结论 Objective:Analysis the change in immune parameters of CD4 、CD8、CD3 related to age and sex in young mice. Methods:Spleen and thymus T cells were separated and FACS assayed the expression of CD3 、CD8、CD4 from mice 3 to 9 weeks of age;Proliferation of splenocytes and thymocytes was measured by CFSE in response to in vitro T-cell receptor-dependent mitogens(ConA and anti-TCR),and to a cell surface-receptor-independent mitogenic combination(PMA+IONO) after cultured 72 h. Results:With regard to distribution of immune cells, no significant sex-related changes were seen in thymocyte expression of CD3 、CD8、CD4 or splenocyte expression of CD3 、CD8、CD4.For splenocytes, significantly more cells were positive for CD3 in 6-9 week old compared with 3-4 week old mice. Thymocyte proliferation was not related to age or sex of the mice. For splenocytes of the 3 weeks old mice, the response to a cell of PMA and ionomycin induced a significantly greater than that by cells from females. For mice 7-8 weeks of age, splenocytes from female mice responded significantly less to stimulation by antibody to TCR.Conclusion:Sexual dimorphism in the immune system may be demonstrated prior to puberty.
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2004年第5期297-299,共3页 Chinese Journal of Immunology
基金 国家自然科学基金资助项目 (3 973 0 410 )
关键词 性别 年龄 T淋巴细胞 Sex Age T lymphocyte
  • 相关文献

参考文献11

  • 1Hirst M.Transitions to informal care in Great Britain during the 1990s[J].J Epidemiol Community Health,2002;56(8):579-587.
  • 2Dulos G J,Bagchus W M.Audrogens indirectly accelerate thymocytes apoptosis[J].Int Immunopharmacol,2001;1(2):321-381.
  • 3Sakabe K,Kawashima J,Seibi K et al.Hormone and immune response,with special reference to steroid hormone(2):sex steroid receptors in rat thymus[J].J Exp Clin Med,1998;15(2):201-211.
  • 4Dominguez-Gerpe L,Rey-Mendez M.Age-related change in primary and secondary immune organs of the mouse[J].Immunol Invest,1998;27:153-165.
  • 5Aspinall R,Andrew D.Gender-related differences in the rats of age associated thymic atrophy[J].Dev Immunol,2001;8(2):95-106.
  • 6Cavallott D,Artico M,Lannetti G et al.Occurrence of adrenergic nerve fibers in human thymus during immune response[J].Neurochem Int,2002;40(3):211-221.
  • 7McCarty M F.Upregulation of lymphocyte apoptosis as a strategy for preventing and treating autoimmune disorders a role for whole-food vegan diets,fish oil and dopamine agonists[J].Med Hypotheses,2001;57(2):258-275.
  • 8Oner H,Ozan E .Effect of gonadal hormones on thymus gland after bilateral ovariectomy and orchidectomy in rats[J].Arch Androl,2002;48(2):115-126.
  • 9Nakayama M,Otsuka K,Sato K et al.Activation by estrogen of the number and function of forbidden T-cell clones in intermediate T-cell receptor cells[J].Cell Immunol,1996;172(2):163-171.
  • 10Kalra R,Singh S P,Kracko D et al.Chronic self-administration of nicrotine in rats impairs T cell responsiveness[J].J Pharmacol Exp Ther,2002;302(3):935-939.

同被引文献18

引证文献3

二级引证文献12

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部