摘要
AIM:To investigate the expression of effector protease receptor-1 (EPR-1),proliferative index Ki-67 and apoptosis index in patients with primary advanced gastric adenocarcinoma and to clarify the significance of EPR-1 expression and its correlationship with the proliferation and apoptosis indexes.METHODS:Using immmunohistochemical staining and terminal deoxynucleotidyl transferase mediated nick end labelling (TUNEL) technique,we determined the expression of EPR-1,proliferative index (Ki-67) and apoptotic index (AI) in 120 paraffin-embedded specimens of primary advanced gastric adenocarcinoma as well as lymph node metastasis and agjacent normal tissues.RESULTS:EPR-1 expression was distributed in the cytoplasm of normal gastric mycoderma,carcinoma cells and smooth muscl cells.The plsitive rate of EPR-1 expression in the primary gastric adenocarcinomas,invasion tumor node and lymph node metastasis was 65.83%,55.29% and 68%,respectively.While the positive rate in normal gastric mycoderma and smooth muscle cells was 46.7% and 53.3%,respectively.The average positive rate of Ki-67 in EPR-1-positive tumors was 7.00% which was significantly lower than that of 8.53% in EPR-1-negative tumors,but the average AI in EPR-1-positive tumors was 1.25%,which was significantly higher than that of 1.00% observed in EPR-1-negative tumors.On the other hand,the average positive labeling index for Ki-67(ki-67) in EPR-1-positive lymph node metastasis was 7.65%,which was significantly lower than that of 9.44% observed in EPR-1-negative lymph node metastasis.However,the average AI in EPR-1-positive lymph node metastasis tumors was 0.99%,which was significantly higher than that of .67% observed in EPR-1-negative lymph node metastasis.CONCLUSION:The frequency of EPR-1 exrpession was significantly higher in primary gastric adenocarcinoma and in its lymph node metastasis than that in normal gastric mucosa.Expression of EPR-1 Score is positively correlated with apoptosis index,but is negatively realted with proliferative index.Thus,Weighted EPR-1 Score and EPR-1 expression in gastric adenocarcinoma cells maybe a potential marker in clinical setting.
AIM:To investigate the expression of effector protease receptor-1 (EPR-1),proliferative index ki-67 and apoptosis index in patients with primary advanced gastric adenocarcinoma and to clarify the significance of EPR-1 expression and its correlationship with the proliferation and apoptosis indexes. METHODS:Using immunohistochemical staining and terminal deoxynucleotidyl transferase mediated nick end labelling (TUNEL) technique,we determined the expression of EPR-1,proliferative index (Ki-67) and apoptotic index (AI) in 120 paraffin-embedded specimens of primary advanced gastric adenocarcinoma as well as lymph node metastasis and adjacent normal tissues. RESULTS:EPR-1 expression was distributed in the cytoplasm of normal gastric mycoderma,carcinoma cells and smooth muscle cells.The positive rate of EPR-1 expression in the primary gastric adenocarcinomas,invasion tumor node and lymph node metastasis was 65.83%,55.29% and 68%,respectively.While the positive rate in normal gastric mycoderma and smooth muscle cells was 46.7% and 53.3%,respectively.The average positive rate of ki-67 in EPR-1-positive tumors was 7.00% which was significantly lower than that of 8.53% in EPR-1-negative tumors,but the average AI in EPR-1-positive tumors was 1.25%,which was significantly higher than that of 1.00% observed in EPR-1-negative tumors.On the other hand,the average positive labeling index for Ki-67 (ki-67) in EPR-1-positive lymph node metastasis was 7.65%,which was significantly lower than that of 9.44% observed in EPR-1-negative lymph node metastasis.However,the average AI in EPR-1-positive lymph node metastasis tumors was 0.99%,which was significantly higher than that of 0.67% observed in EPR-1- negative lymph node metastasis. CONCLUSION:The frequency of EPR-1 expression was significantly higher in primary gastric adenocarcinoma and in its lymph node metastasis than that in normal gastric mucosa.Expression of EPR-1 was significantly correlated with tumor histological subtypes and tumor differentiation. Weighted EPR-1 Score is positively correlated with apoptosis index,but is negatively related with proliferative index. Thus,Weighted EPR-1 Score and EPR-1 expression in gastric adenocarcinoma cells maybe a potential marker in clinical setting.
