摘要
AIM: To explore the value of avidin-biotin system enzyme-linked immunosorbent assay (ABC-ELISA) in diagnosis of intestinal acariasis.METHODS: Mite-specific IgG levels in serum of 48 patients with intestinal acariasis were measured with ABC-ELISA.The sensitivity of this method was compared with that of staphylococcal protein A enzyme-linked immunosorbent assay (SPA-ELISA).RESULTS: The positive rate of mite-specific IgG detected with ABC-ELISA and SPA-ELISA was 89.58% (43/48) and 56.25% (27/48), respectively. The positive rate with ABC-ELISA was statistically higher than that with SPA-ELISA (x^2= 13.50, P<0.01).CONCLUSION: ABC-ELISA is an effective method for the diagnosis of intestinal acariasis.
AIM:To explore the value of avidin-biotin system enzyme- linked immunosorbent assay (ABC-ELISA) in diagnosis of intestinal acariasis. METHODS:Mite-specific IgG levels in serum of 48 patients with intestinal acariasis were measured with ABC-ELISA. The sensitivity of this method was compared with that of staphylococcal protein A enzyme-linked immunosorbent assay (SPA-ELISA). RESULTS:The positive rate of mite-specific IgG detected with ABC-ELISA and SPA-ELISA was 89.58% (43/48) and 56.25% (27/48),respectively.The positive rate with ABC- ELISA was statistically higher than that with SPA-ELISA (x^2=13.50,P<0.01). CONCLUSION:ABC-ELISA is an effective method for the diagnosis of intestinal acariasis.
