口腔扁平苔藓者白色念珠菌检出率及分离株的磷脂酶活性研究

The oral Candida albicans isolation rate of patients with OLP and phospholipase activity of isolates

目的 :研究健康人和口腔扁平苔藓 (orallichenplanus,OLP)患者的口腔白色念珠菌检出率及分离株的磷脂酶活性。方法 :采用含漱液浓缩培养法及白色念珠菌微生物学鉴定法 ,考察健康对照组、糜烂型和非糜烂型OLP患者的白色念珠菌检出率 ;应用蛋黄培养基沉淀环面积测量法 ,比较来自于健康人 (2 6株 )、糜烂型OLP患者 (6 2株 )以及非糜烂型OLP患者 (2 4株 )共 112株白色念珠菌的磷脂酶活性大小。结果 :糜烂型OLP患者组的口腔白色念珠菌检出率高于健康对照组和非糜烂型扁平苔藓患者组 ;糜烂型和非糜烂型OLP患者组的白色念珠菌分离株的磷脂酶活性高于健康对照组。结论 :两种类型的OLP患者组的白色念珠菌分离株与健康对照组相比 ,具有不同的毒性特征 ,白色念珠菌与OLP(尤其是糜烂型 )发生发展可能相关。

Objective:To investigate the oral Candida albicans isolation rate of patients with OLP and phospholipase activity of isolates.Methods:An oral rinse method and a series of identification assays(including Gram staining,germ tube test,45 ℃ growth test and API 20C AUX test) were used to identify the isolates of Candida albicans.A total of 112 isolates,compromising isolated from healthy control(26),erosive OLP (62) and nonerosive OLP(24),were screened for the phospholipase activity by using the in vitro plate assay method.Results:i)The Candida albicans isolation rate of the erosive OLP group(44.29%) was higher than that of nonerosive OLP(15.00%) or healthy control group(20.31%).ii)The phospholipase activity of the isolates from erosive and nonerosive OLP groups was higher than that of those from healthy controls,while no significant difference was found between the isolates from erosive and nonerosive OLP groups.Conclusion:Candida albicans,some isolates with special virulence attributes may contribute to the development and progression of OLP(ecpecially to that of erosive OLP).