经腹膜外盆腔淋巴间隙给予IL-2对妇科肿瘤患者TIL亚群及NK细胞的影响

Study on the TIL and NK of IL-2 Injected via Pelvic Retroperitoneal Space in Gynecological Cancer Patient

目的 探讨通过盆腔腹膜外淋巴间隙进行肿瘤生物治疗的可行性。方法 妇科恶性肿瘤患者 4 1例 ,随机分为 IL- 2组 (n=10 )、IL- 2 +5 - Fu组 (n=11)、5 - Fu组 (n=10 )及对照组 (n=10 ) ;通过经盆腔腹膜外淋巴间隙留置管 ,分别予以 IL- 2、IL- 2 +5 - Fu、5 - Fu治疗后 ,手术中摘取肿瘤组织 ,分离浸润淋巴细胞 ,用流式细胞仪检测 T细胞表面标记、CD2 5活化标记变化及 NK细胞数量。结果 治疗后各治疗组肿瘤浸润淋巴细胞 CD3+、CD4 +、CD8+ 、CD2 5 + 、NK细胞数量均明显高于对照组 (P值均 <0 .0 5 ) ;IL - 2 +5 - Fu组 CD3+ 、CD4 + 、CD8+ 、CD2 5 + T细胞含量及 NK细胞数量明显高于 5 - Fu组 ,IL - 2组 CD2 5 + T细胞含量及 NK细胞数量明显高于 5 - Fu组 (P<0 .0 5 )。结论 经盆腔腹膜外淋巴间隙进行生物治疗能有效激活机体 T细胞反应 ,促进 T细胞增殖、活化和浸润 ,增强患者免疫系统对肿瘤的有效防御反应。

Objective To verify the feasibility and effect of biotherapy instituted via pelvic retroperitoneal space on gynecological cancer. Methods Injecting IL-2 (and/or) 5-Fu through a tube installed in the pelvic retroperitoneal space. Counting the subpopulation of T cell and NK of lymph-nodes of pelvis after the drugs being by FCM. Results The numbers of CD3 +, CD4 +, CD8 +, CD25 + and NK cells in treatment group were significantly higher than those in the control group. And the numbers of these cells in the IL-2+5-Fu group were significantly higher than those in the 5-Fu group. The CD25 + and NK cell nubmers in the IL-2 group were significantly higher than those in the 5-Fu group(P<0.05). Conclusion The IL-2 injected via pelvic retroperitoneal space can promote the activity,development and infiltrating of T cell and NK cell in the tumor tissue.