膀胱移行上皮Toll样受体分子诱导表达的实验研究

Study on the Inducible Expression of Toll-like Receptors in Human Bladder Cell Line

目的 了解膀胱移行上皮在大肠杆菌和卡介苗的不同刺激下 Toll样受体分子 (TL Rs)的表达。方法用不同剂量 (菌数 /细胞数比值 )的大肠杆菌和卡介苗 (BCG)分别刺激培养人膀胱移行上皮癌 T2 4细胞 ,1h后以RT- PCR检测其 TL R- 2和 TL R- 4的 m RNA水平。结果 膀胱癌 T2 4细胞有 TL R- 2和 TL R- 4表达 ;大肠杆菌各剂量组 T2 4细胞 TL R- 2和 TL R- 4的表达水平与未刺激组相比无显著差异 ;BCG刺激后 T2 4细胞中 TL R- 2和TL R- 4的表达水平随 BCG剂量的增加而逐渐升高 ,在菌数 /细胞数比值为 10时达最高 ,随后有所降低。结论 膀胱移行上皮在大肠杆菌和 BCG等不同微生物的刺激下 ,其 Toll样受体分子的表达存在差异。

Objective To investigate and compare the expression of Toll-like receptors (TLRs) in human bladder cells under the stimulus of Escherichia coli (E.coli) versus that under the stimulus of Bacillus Calmette-Guérin(BCG). Methods Human bladder cancer cell line T24 was cultured for 1 hour under the stimuli of various doses (bacillus-cell ratio) of E.coli and BCG respectively. The levels of expression of TLR-2 and TLR-4 mRNA in T24 cells were assessed by RT-PCR method. Results By comparison with control, there was no difference observed on the expression of either TLR-2 or TLR-4 in T24 cells under the stimulus of E.coli. The expression level of either TLR-2 or TLR-4 was increased under the stimulus of BCG in a dose-dependent manner. The effects reached the highest level when the dose of BCG was 10 bacilli per cell. Conclusion There exist different expression patterns of TLRs in bladder transitional cells under the different stimuli of E. coli and BCG respectively.