雷公藤多甙对实验性重症肌无力动物骨骼肌复合动作电位的影响

The Effect of Tripterygium Wilfordii Polyglycosidium on the Compound Action Potential of Skeletal Muscle in Experimental Animals with Myasthenia Gravis

目的 观察雷公藤多甙 (TWP)对实验性自身免疫性重症肌无力 (EAMG)动物肌肉活动的直接效应。方法 将筛选出的 EAMG小鼠腓肠肌和旋前圆肌标本置于 TWP恒温浴液中 ,观察给药前后骨骼肌复合动作电位衰减百分率变化。结果 14 0 mg/ L TWP对 EAMG小鼠腓肠肌复合动作电位衰减百分率有明显影响 (P<0 .0 5 ) ,用药 1.5 h后衰减百分率明显降低〔用药前 (14± 36 ) % ,用药后 (- 4 0± 2 4 ) %〕;2 4 0 m g/ L TWP对 EAMG小鼠旋前圆肌复合动作电位衰减百分率有明显影响 (P<0 .0 5 ) ,用药 1.5 h后衰减百分率明显降低〔用药前 (17±2 5 ) % ,用药后 (- 17± 10 ) %〕;340 mg/ L TWP对 EAMG小鼠腓肠肌和旋前圆肌复合动作电位衰减百分率的影响无明显差异。结论 TWP具有直接增强 EAMG动物骨骼肌兴奋收缩的效应。

Objective To observe and analyze the direct effect of tripterygium wilfordii polyglycosidium (TWP) on the activity of skeletal muscle in experimental animals with myasthenia gravis(EAMG). Methods C57BL/6 mice were used. After sieving EAMG animals, we observed and compared the variation of the attenuation percentage of compound action potential of gastrocnemius muscle and pronator teres muscle of EAMG before and after the muscle specimens being immersed in the breeding ground of TWP solution. Such variation of attenuation percentage was taken as an indicator of the direct effect of TWP on skeletal muscle. Results ① Evident influence of TWP on the attenuation percentage of compound action potential of gastrocnemius muscle was observed(P<0.05), and apparent decrease of the attenuation percentage could be seen one and a half hours after the muscle was immersed 〔(14±36)% before treatment, (-40±24)% after treatment〕; ②The influence of TWP on the attenuation percentage of compound action potential of pronator teres muscle was noted(P<0.05), and an apparent decline of the attenuation percentage could be observed one and a half hours after the muscle was immersed〔(17±25)% before treatment, (-17±10)% after treatment〕; ③There was no distinctive difference between the gastrocnemius muscle and pronator teres muscle in respect of their attenuation percentages of compound action potential. Conclusion TWP has direct remedial effect on the activity of skeletal muscle of EAMG.