青梅感冒冲剂中的五指柑和青蒿的薄层色谱鉴别

TLC identification of Folium Viticis negundo and Herba Artemisiae annuae in Qinmei cold granules for oral suspension

目的:研究青梅感冒冲剂中五指柑、青蒿的薄层色谱鉴别方法。方法:分别取五指柑药材、青蒿药材、青梅感冒冲剂及阴性制剂的乙酸乙酯提取液,在硅胶薄层板上五指柑以ψ(甲苯:丙酮)=8:2为展开剂展开,青蒿以ψ(苯:乙酸乙酯:水)=5:2:0.1为展开剂展开,在紫外灯(365 nm下)检视。结果:在两者供试品色谱中与各自对照药材色谱相应位置上,均有相应的斑点,阴性制剂色谱图中无此斑点。结论:该法专属性强,为该制剂增加质量控制指标提供了实验依据。

Objective: To study the TLC identification of Folium vilicis Negundo ( FVN) and Herba artemisiae Annuae ( HAA) in Qinmei cold granules for oral suspension. Methods; The extracts of FVN, HAA and its preparation were subjected to TLC in different development systems, (toluene : acetone) =8 : 2 and (benzene : acetic ether : water) =5 : 2 : 0. 1 were used as development reagents for FVN and HAA respectively. Results; Characteristic spots could be observed at 365 nm on positions corresponding to FVN, HAA and the preparation respectively, while the negative control showed no spots. Conclusion: This method is highly exclusive and can be used in the quality control of the preparation.