高效毛细管电泳法测定阿莫西林口服混悬剂中阿莫西林的含量

Determination of amoxycillin in amoxycillin oral suspension by HPCE

目的:测定阿莫西林口服混悬剂中阿莫西林的含量。方法:高效毛细管电泳法。毛细管60cm×75μm,运行缓冲液30 mmol/L硼砂(pH 9.2),高压进样5 s,分离电压12 kV,温度为25℃,检测波长254 nm,阿魏酸为内标。结果:阿莫西林在6～30 μg/mL质量浓度范围内线性关系良好(r=0.9996),平均回收率99.10％(n=5,RSD=1.89％)。结论:本法简单、快捷、灵敏。

Objective: to determine the content of amoxycillin in amoxycillin oral suspension. Methods: HPCE was used. The best condition was achieved with a fused silca capillary tube (60 cm × 75μm) , 30 mmol/L sodium tetraborate decahydrate buffer ( pH 9. 2) , at a constant voltage of 12 kV and temperature at 25℃. The UV detection wavelength was at 254 nm and ferulic acid was determinaed with internal standard method. Results: the calibration curves was linear in the range of 4 -20 μg/mL for amoxycillin( r = 0. 9997) . The average recovery of amoxycillin was 99. 10% ( n =5 ,RSD= 1. 89% ) . Conclusions: this method is simple, quick and sensitive.