RP-HPLC法测定不同品种和产地红景天中指标成分的含量(英文)

Determination of marker compounds in plants of Rhodiola L. from different habitats by RP-HPLC

目的 建立快速、有效的反相高效液相 (RP- HPL C)检测方法 ,测定不同品种和产地红景天中红景天苷、红景天芬、酪醇、红景天素、红景天任的含量 ,以评价红景天的质量。方法 方法 :甲醇 -水 (0 .5 m mol/ L SDS的 1%醋酸水溶液 )检测红景天苷 ;方法 :乙腈 -水检测红景天芬 ;方法 :乙腈 -磷酸水溶液梯度洗脱同时检测 5个有效成分。结果 红景天苷的含量为 0 .0 2 1%～ 1.4 2 0 % ;红景天芬除在蔷薇红景天和高山红景天中含量较高外 ,在其他品种中均非常低或低于检测限 ;5个有效成分的含量因品种或产地的不同而有很大的变化。结论 建立的 3种 RP-HPL C方法可同时测定红景天苷、红景天芬等 5个有效成分。综合考虑不同品种中 5个有效成分的含量 ,以产自新疆的蔷薇红景天和吉林的高山红景天为国内较好的品种。

ObjectTo develop the reliable RP-HPLC methods for the determination of salidroside, tyrosol, rosavin, rosin, and rosarin in the plants of Rhodiola L. and to evaluate their species from different habitats. Methods Method Ⅰ: methanol-water (0.5 mmol/L SDS in 1% acetic acid aqueous solution) system for the analysis of salidroside; method Ⅱ: acetonitrile-water system for rosavin; method Ⅲ: aqueous acetonitrile-phosphoric gradient system for salidroside, tyrosol, rosavin, rosin, and rosarin. Results The contents of salidroside in different species range from 0.021% to 1.420%, and those of rosavin in all species are very limited or undetected except in Rhodiola rosea L. and R. sachalinensis. The contents of the five marker ingredients are significantly species- and habitat-dependent. Conclusion Three RP-HPLC methods are established for quantitative analysis of the above five marker ingredients in the meantime, respectively. Evaluation of the quality of varied species of Rhodiola L. shows that R. rosea growing in Xinjiang Uygur Autonomous Region and R. sachalinensis growing in Jilin province are the two better species contained with abundant above-mentioned ingredients in China.