牛膝的rDNAITS序列分析

Ribosomal rDNA ITS sequence analysis of root of Achyranthes bidentata

目的 探讨不同产地牛膝的 ITS的序列变异 ,为鉴别牛膝提供 DNA分子标记。方法 利用特异性 PCR技术对牛膝的 r DNA ITS区碱基序列进行测定 ,报道了牛膝的 r DNA ITS区的碱基序列。结果 得到核糖体 DNA中的 ITS及 5 .8S r DNA完全序列 ,18S和 2 6 S r DNA部分序列 ,共约 6 5 0 bp。牛膝与川牛膝及土牛膝碱基序列有明显差异 ,不同产地、不同栽培品种牛膝碱基序列无差异。结论 此法可用于牛膝种间及真伪品鉴别。

ObjectTo study the correlation between ITS sequence variation of root of Achyranthes bidentata Blume from different habitat to provide their DNA molecular marker for identification of A. bidentata. Methods To determine rDNA ITS base sequence by peculiar PCR technology. Results The completed sequence of ITS and 5.8 S rDNA, and the partial sequence of 18 S rDNA and 26 S rDNA of root of A. bidentata were obtained. They are about 650 bp. There is obvious diversity among the roots of A. bidentata, Cyathula officinalis Kuan, and A. aspera, while there is no difference among the roots of A. bidentata from different habitat and by different cultivacation breeds. Conclusion The method can be used to identify the root of A. bidentata among different species and to differentiate its fakes.