改性聚β-羟基丁酸脂与人肾上腺细胞的生物相容性研究

Biocompatibitity of modified PHB to adrenocortical cells

目的 探讨聚 β 羟基丁酸脂 (PHB)的生物相容性和PHB改性方法。 方法 以明胶作为改性材料对PHB进行改性 ,并与人肾上腺细胞共同培养 ,通过形态学、细胞增殖和细胞分泌三方面来评价改性PHB的生物相容性。结果 PHB对肾上腺细胞无毒性 ,对细胞增殖和分泌功能无影响 (P均 >0 .0 5 )。结论 PHB是一种较为理想的细胞载体材料 ,可应用于肾上腺细胞移植。

Objective To investigate the biocompatibitity of modified PHB to adrenocortical cells.Methods Using gelatin as a modified materials,PHB was retrofited and adreno-cortical cells were seeded to the modified PHB.The effect of PHB on the proliferation and secreting function of adrenocortical cell were evaluated by MTT and CLIA method.Results Adrenocortical cells were able to grow and survive on PHB.No effect on the proliferation and secreting fucntion of adrenocortical cells observed.Conclusion The modified PHB is better and more ideal biodegradable material as cell carrier for transplantation of adrenocortical cells.