多器官功能不全综合征大鼠结肠运动功能变化和机制的研究

Changes of colon motility and mechanism in rats with multiple organ dys function syndrome

目的 大鼠多器官功能不全综合征 (MODS)结肠动力变化并探讨其机制。方法 将Wistar大鼠分成 2组 :正常对照组和MODS模型组。观测 2组大鼠 1h排便的粪点数、2组结肠平滑肌环行肌条振幅、平滑肌细胞长度及钙离子的变化 ;测定平滑肌细胞三磷酸肌醇 (IP3 )和血清一氧化氮 (NO)含量的变化。结果 在 10 -4mol/L乙酰胆碱和 10 -1mol/L氯化钾的作用下 ,MODS组肌条收缩振幅小于对照组。正常对照组平滑肌细胞的长度分别是 (4 9.1± 11.8) μmol和 (69.2±15 .7) μmol ;MODS模型组平滑肌细胞的长度分别是 (65 .1± 18.5 ) μmol和 (87.2± 2 3 .7) μmol。正常对照组各组数据与MODS模型组相应的数据相比有明显差别 (P <0 .0 5 )。MODS模型组粪点数、钙离子浓度和IP3 含量较正常对照组低 ;而NO较正常对照组高 (P <0 .0 5 )。结论 MODS情况下 ,结肠平滑肌的运动功能减弱与平滑肌胞浆钙离子和平滑肌细胞IP3 含量降低以及NO增加有关。

Objectice To explore colon motility changeand its mechanism in the rats with multiple organ dysfunction syndrome (MODS).Methods Forty Wistar rats were divided two groups:control group and MODS group.The number of stool,the amplitude change of circular muscle strips,the length of cell and [Ca 2+]i of colonic smooth muscule,the change of IP 3 and serum NO were observed in the two groups.Results The number of stool,[Ca 2+]i and IP 3 in the MODS group were lower than in the control group,but NO in the MODS group was higher than in the control group (P<0.05).After using 10 -4 mol/L acetylcholine (Ach) and 10 -1 mol/L potassium chloride respectively,the cell length of smooth muscle was (49.1±11.8) μmol/L and (69.2±15.7) μmol/L in the control group and (65.1±18.5) μmol/L and (87.2±23.7) μmol/L in the MODS group respectively.The data of the control group were obviously different from the MODS group (P<0.05).Conclusion Colon motility was weakened in MODS condition by reducing [Ca 2+]i and IP 3 of smooth muscle cell and increasing serum NO.