SD大鼠视神经不同程度损伤的闪光视觉诱发电位动态监测

Investigation on flash-visual evoked potentials of SD rats after injuries to optic nerve in different grades

目的 探索大鼠视神经不同程度损伤后闪光视觉诱发电位 (F VEP)的变化特征 ,为进一步研究间接性视神经损伤的修复提供实验基础和依据。方法 利用压力恒定的反向镊分别夹持大鼠视神经 6、30、6 0s,建立轻、中、重度视神经不完全损伤模型 ,记录正常及损伤后F VEP潜伏期和振幅的变化。结果 正常大鼠F VEP波形稳定 ,主波潜伏期 (L5b)为 5 3 6 7± 3 12ms,振幅 (A5 )为 17 83±5 91μV。视神经损伤后的大鼠F VEP与正常大鼠比较差异有统计学意义 (P <0 0 1)。损伤程度不同 ,F VEP的改变不同 ,损伤程度越重 ,伤后时间越长 ,潜伏期延迟越显著 ,波幅降低程度越大 ,F VEP的熄灭越早。结论 大鼠视神经损伤后F VEP的变化规律与神经的损伤程度一致 ,F

Objective To explore the characteristic and the rule of flash-visual evoked potentials (F-VEP) of SD rats after optic nerve injury in different grades. Methods By using APS-2000 multifunctional electric physiological instrument, changes in F-VEP were observed in normal optic nerve and injured optic nerve (crushed in 3 grades) in rats. The latency and amplitude of F-VEP wave, were recorded and statistically. Results The F-VEP wave was reliably displayed with characteristic curve. The L5b was(53.67±3.12)ms and A5 was(17.83±5.91)μv. The latency of F-VEP in rats with optic nerve injury in different grades showed a significant prolongation, and the amplitude showed a significant decrease compared with normal rats (P<0.01). The abnormity of F-VEP was in accord with the injury degree. Conclusion The results demonstrated that the F-VEP was reliable and objective in reflecting the changes in the optic nerve function of SD rats after different degrees of injury. It provided experimental base data for the further study on treatment of optic nerve injury.