17β-雌二醇对人涎腺黏液表皮样癌Mc3增殖周期的影响

Effect of 17β-estradiol on the proliferation cycle of human mucoepidermoid carcinoma Mc3 cells

目的 探讨 17β 雌二醇 (E2 )对体外培养的人涎腺黏液表皮样癌Mc3细胞系增殖周期的影响。方法 以不同浓度、时间的E2 处理Mc3细胞 ,采用MTT法、流式细胞术、免疫组织化学染色法 ,检测E2 对人涎腺黏液表皮样癌Mc3细胞系细胞群体倍增时间、细胞周期分布以及对CyclinD1、P2 7Kip1阳性表达的影响。结果 浓度分别为 10 -9、10 -8、10 -7mol/L的E2 作用于细胞第 5天时 ,MTT法测得E2 处理组细胞增殖促进率分别为 2 9%、5 4 %、5 9% ,CyclinD1的阳性表达率分别增加了 16 %、9%、2 4 % ,P2 7Kip1的阳性表达率分别减少了33%、30 %、5 5 %。流式细胞术检查结果显示 ,10 -7mol/L的E2 处理 12、18、2 4h后 ,细胞周期中S期细胞比相应对照组分别增加了 11 3%、6 6 %、4 6 7% ,细胞增殖指数分别增加了 6 0 %、3 6 %、2 0 5 5 %。结论 生理浓度的E2 可以促进CyclinD1的表达、降低P2 7Kip1的阳性表达 ,从而促进细胞周期G1/S期转换 ,增加S期细胞数量 ,加速DNA合成 ,刺激肿瘤发生。

Objective To investigate the effect of 17β-Estradiol(E 2)on the proliferation cycle of human mucoepidermoid carcinoma Mc3 cells. Methods Mc3 cells were processed with various concentrations of E 2 for various durations. Then, cell proliferation and cell cycle distribution were examined by MTT assay and flow cytometry; CyclinD1 and P27 Kip1 expressions were assessed with immunohistochemistry. Results After being exposed to E 2 in concentrations of 10 -9,10 -8 or 10 -7mol/L for 5 days, the proliferation of the Mc3 cells was enhanced by 29%,54% and 59% respectively, CyclinD1 expression was increased by 16%,19% and 24%, And P27 Kip1 expression was decreased by 33%,30% and 55%, respectively. Compared with the control group, the number of cells in S phase increased by 11.3%,6.6% and 46.7% after the Mc3 cells were exposed to E 2 in the concentration of 10 -7mol/L for 12h,18h or 24h, and the proliferation index(PI) was also increased by 6.0%,3.6% and 205.0% respectively. Conclusion Physiological concentration of E 2 may promote Mc3 proliferation by increasing the expression of CyclinD1 and decreasing the expression of P27 Kip1, thereby stimulate the growth and development of the tumor.