乙型肝炎病毒核心基因转基因小鼠的建立

Establishment of transgenic mice for hepatitis B virus core gene expression

目的 :建立乙型肝炎病毒核心基因转基因小鼠。方法 :构建乙型肝炎病毒核心基因真核表达载体pcDNA3 HBc ;采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中 ,然后将注射存活的受精卵植入假孕母鼠的输卵管 ;取其产 1 0d龄子代鼠进行鉴定。结果与结论 :表达载体内切酶酶切及序列分析结果与预期一致 ;PCR鉴定转基因阳性小鼠比例为 2 5 % (6 / 2 4 )。

Aim: To establish transgenic mice for hepatitis B virus core gene expression. Methods: The HBV core gene eukaryotic expression vector was constructed and the linerized DNA was microinjected into the male pronuclei of C57BL/6 mouse fertilized eggs. The eggs survived from microinjection were transplanted into oviducts of pseudopregnant recipients. The transgenic mice were identified by PCR. Results and Conclusion: The map of endonuclease digestion and sequencing of the eukaryotic expression vector was consistent with what in anticipation. The transgene positive mice accounted for 25%(6/24). The transgenic mice for hepatitis B virus core gene expression are established successfully.