脑肽精对阿尔茨海默病大鼠脑中NO、NOS和AChE的影响

Effects of brain peptide crystal on NO,NOS,and AChE in the brain of rat models with Alzheimer's disease

目的 :观察脑肽精 (BPC)对阿尔茨海默病 (AD)大鼠脑中一氧化氮 (NO)、一氧化氮合酶 (NOS)和乙酰胆碱酯酶 (AChE)含量的影响。方法 :雄性SD大鼠 84只 ,随机分成 7组 ,每组 1 2只。Ⅰ～Ⅶ组分别为正常对照组 ,AD模型组 ,AD模型 +生理盐水组 ,AD模型 +脑复康 0 .3g/kg治疗组 ,AD模型 +BPC 1 5mg/kg治疗组 ,AD模型 +BPC30mg/kg治疗组及AD模型 +BPC 6 0mg/kg治疗组。向SD大鼠的海马注射 5 μg鹅膏蕈氨酸 (ibotenicacid ,IBO)建立AD模型后 ,分别用生理盐水、BPC或脑复康连续灌胃 2 0d治疗 ,1次 /d ,每次 2ml。灌胃期满后将大鼠断头处死 ,并立即在冰盘中开颅取脑做成组织匀浆。离心取上清液检测NO、NOS和AChE的含量。结果 :①与Ⅰ组比较 ,Ⅱ组大鼠脑中NO、NOS含量均明显升高 (P <0 .0 5 ) ,但AChE显著降低 (P <0 .0 5 ) ;②与Ⅱ组比较 ,Ⅲ组大鼠脑中的NO、NOS、AChE均无明显改变 (P >0 .0 5 ) ;③与Ⅱ组比较 ,脑复康组及BPC各浓度组大鼠脑中NO、NOS的含量均显著降低 (P <0 .0 5 ) ,AChE无明显改变 (P >0 .0 5 ) ;④BPC各浓度组NO、NOS间差异均有统计学意义 (P <0 .0 5 ) ,AChE无明显改变 (P >0 .0 5 )。结论 :BPC能够显著降低AD模型大鼠脑中NO。

Aim:To investigate the effects of brain peptide crystal (BPC) on the levels of nitric oxide (NO), nitric oxide synthase (NOS) and acetylcholinesterase(AChE) in the brain of the rat models of non hereditary Alzheimer's disease(AD). Methods: Eighty four healthy male SD rats (8～12 weeks, 170～210 g) were randomly divided into 7 groups, 12 rats is each group. AD animal model was established by injecting 5 μg ibotenic acid(IBO) into rat brain to damage bilateral nbM.Group I was normal control, groupⅡ was AD animal model, group Ⅲ was the one of AD model rats treated by normal saline, group Ⅳ was AD animal model treated by Naofukang 0.3 g/(kg·d), groupⅤ was AD animal model treated by BPC 15 mg/(kg·d), group Ⅵ was AD animal model treated by BPC 30 mg/(kg·d), group Ⅶ was AD animal model treated by BPC 60 mg/(kg·d).The animals in groups Ⅲ,Ⅳ,Ⅴ,Ⅵ,and Ⅶ were treated for 20 days. Then, all the animals were decapitated ,and the brain was homogenized and centrifuged. The supernatant was used to analyze the contents of NO, NOS and AChE. Results: Compared with normal control group, the contents of NO and NOS in groupⅡ had a significant increase ( P < 0.05), and that of AChE had a marked decrease( P <0.05); compared with group Ⅱ, the levels of NO, NOS,and AChE in groupⅢ did not change significantly ( P >0.05);comparing with groupⅡ, the levels of NO and NOS in groupsⅤ,Ⅵ,and Ⅶ, decreased significantly ( P <0.05), and that of AChE did not change significantly( P > 0.05). The levels of NO and NOS both had significant difference( P <0.05) among groupsⅤ,Ⅵ,and Ⅶ,while that of AChE had not ( P >0.05). Conclusion: BPC can decrease the levels of NO and NOS in brain of AD model rats.