NS-398对人胃癌裸鼠移植瘤血管生成的影响

Effects of NS-398 on the angiogenesis of gastric carcinoma xenograft in nude mice

目的 :探讨NS 398对裸鼠胃癌移植瘤血管生成的影响。方法 :1 2只裸鼠随机分为 2组 ,以人胃癌细胞株SGC790 1建立荷瘤裸鼠动物模型。治疗组于接种第 2d开始给予 1 0mg/kgNS 398腹腔注射 ,隔d给药 ;对照组隔d腹腔注射同体积PBS ,连续 2 0d ,观察抑瘤率 ,应用免疫组织化学技术检测 2组肿瘤组织环氧合酶 2 (COX 2 )、VEGF表达及微血管密度 (MVD)。结果 :NS 398抑瘤率达 88.7% ,COX 2、VEGF及MVD在治疗组分别为 2 .6± 0 .8,2 .3± 1 .2和 2 1 .2± 4 .8,显著低于对照组的 5 .0± 0 .6 ,5 .8± 0 .4和 4 1 .3± 2 .6 (P均 <0 .0 5 )。结论 :NS 398可能通过抑制COX 2。

Aim:To investigate the effects of NS 398 on the angiogenesis of gastric carcinoma xenografts in nude mice. Methods:Twelve female athymic mice inoculated with human gastric cancer cell line SGC7901 subcutaneously were randomly divided into two groups. The treatment group received alternate day NS 398 solution at a dose of 10 mg/kg intraperitoneally and control mice received the same volume of PBS.After 20 consecutive days,cyclooxygenase 2 (COX 2), VEGF expression and microvascular density (MVD) of the xenograft tissue were detected by immunohistochemical staining. Results: COX 2 ,VEGF and MVD were 2.6±0.8, 2.3±1.2 and 21.2 ±4.8 in carcinoma tissues treated with NS 398, which were higher than those(5.0±0.6,5.8±0.4 and 41.3±2.6,respectively) in control group ( P < 0.05). Conclusion: NS 398 may suppress angiogenesis of gastric carcinoma xenografts in nude mice via inhibiting COX 2 and reducing the expression of VEGF.