川芎嗪对新生大鼠离体胰岛分泌胰岛素的影响

Effects of ligustrazine on insulin secretion of pancreatic islets in vitro in new-born rats

目的 :观察川芎嗪对新生大鼠离体胰岛胰岛素分泌的影响并探讨其作用途径。方法 :利用放射免疫技术 ,分别测定不同浓度川芎嗪处理组 ,川芎嗪处理不同时间组及不同浓度维拉帕米与川芎嗪共同处理组培养的大鼠离体胰岛的基础胰岛素分泌量和高糖刺激的胰岛素分泌量。结果 :①川芎嗪 1 .0mmol/L、2 .0mmol/L、4 .0mmol/L作用于胰岛 2 4h ,其基础胰岛素分泌量明显高于对照组 (P <0 .0 5 ) ,作用呈剂量依赖性 ,而高糖刺激的胰岛素分泌量无明显改变 (P >0 .0 5 ) ;②川芎嗪 1 .0mmol/L分别作用于胰岛 1 2h、2 4h、36h后 ,基础胰岛素分泌量高于对照组(P <0 .0 5 ) ,呈一定的时间依赖性 ;③维拉帕米 2 5 μg/L、5 0 μg/L、1 0 0 μg/L与 2 .0mmol/L川芎嗪联合作用后基础胰岛素分泌量低于对照组 (P <0 .0 5 ) ,且呈剂量依赖性。结论 :川芎嗪能促进离体培养大鼠胰岛基础胰岛素分泌 。

Aim:To study the effects of ligustrazine on insulin secretion in cultured new born rat pancreatic islets. Methods:The new born rat pancreatic islets were isolated and cultured. The contents of insulin in culture medium, which had been treated by different experimental agent for different time according to study design, were measured through the way of radio immunoassay. Results: The basal insulin secretion of cultured new born rat islets treated by ligustrazine for 24 h at a dose of 1.0 mmol/L, 2.0 mmol/L, and 4.0 mmol/L was significantly higher than that of control group ( P<0.05, n = 6), and the action of ligustrazine on insulin secretion was in a dose dependent manner. However, ligustrazine had no significant effects on glucose stimulated insulin secretion ( P>0.05, n =6) in the same circumstance; the pancreatic islets treated by ligustrazine (1.0 mmol/L) for 12 h, 24 h and 36 h, respectively, led to a significant increase of basal insulin secretion in a time dependent manner compared with control group ( P<0.05, n =6); the pancreatic islets treated by verapamil, a blocker of Ca 2+ channel, for 24 h at a dose of 25 μg/L, 50 μg/L, 100 μg/L dose dependently diminished the effect of ligustrazine( P<0.05, n =6).Conclusion: Ligustrazine, probably as an agonist of Ca 2+ channel in pancreatic islet B cell, can increase the basal insulin secretion of cultured new born rat pancreatic islets.