上皮性卵巢癌PTEN启动子CpG岛甲基化研究

Study on the Promoter Methylation of FTEN Gene in Epithelial Ovarian Carcinoma

目的探讨上皮性卵巢癌中抑癌基因PTEN启动子CpG岛的甲基化情况。方法选用甲基化敏感性核酸内切酶HpaⅡ酶切-PCR法,检测64例上皮性卵巢癌组织(浆液性卵巢襄47例,粘液性卵巢癌17例)以及12例正常卵巢组织中PTEN启动子CpG岛甲基化情况。结果正常卵巢组织、浆液性卵巢癌组织和粘液性卵巢癌组织中PTEN启动子甲基化阳性率分别为8.33％(1/12)、57.45％(27/47)和47.06％(8/17),浆液性和粘液性这两种不同组织学类型的上皮性卵巢癌之间的差异无显著性(P<0.05),但均与正常组织间有显著性差异(P分别<0.01,0.05)。结论 PTEN启动子CpG岛甲基化在浆液性卵巢癌和粘液性卵巢癌中均有很高的发生率,提示PTEN基因启动子异常甲基化可能是其在卵巢癌中主要灭活机制之一。

Objective To explor the promoter methylation of PTEN gene in human epithelial ovarian carcinoma.Methods The promoter methylation of PTEN gene CpG islands from 47 cases of serous cystadenocarcinoma,17cases of mucinous cystadenocarcinoma and 12 of normal subjects was analyzed by using methylation sensitive endonuclease Hpa II-PCR method.Results Promoter of PTEN gene was methylated in 8.33%(l/12)of normal subjects,57.45% (27/47)of serous cystadenocarcinoma,and 47.06% (8/17)of mucinous cystadenocarcinoma.The promoter methylation of PTEN gene in both serous cystadenocarcinoma and mucinous cystadenocarcinoma is much higher as compared with normal o-varian n皊sus(P<0.01,0.05,respectively),and no difference between serous cystadenocarcinoma and mucinous cystandenocarnoma was observed (P> 0.05).Conclusion The promoter methylation of PTEN gene CpG islands occurs very common in both serous cystadenocarcinoma and mucinous cystaenocarcinoma,indicating that abnormal promoter methylation is one of the prodominant inactivation mechanismi of the PTEN gene and may be associated with pathogenesis of human ovarian carcinoma.