摘要
本文报道了小剂量LaCl_3、SmCl_3及YbCl_3对体外培养细胞的长期作用。实验结果表明应用含LaCl_3、SmCl_3及YbCl_3终浓度分别为0.01mmol/L的RPMI1640液培养细胞16个月后对细胞无毒性作用;应用~3H-TdR脉冲标记放射自显影术证明它们对培养细胞的分裂活性、DNA合成略有促进作用;扫描电镜观察,细胞表面结构无改变;透射电镜观察也未见细胞内部结构上的变化;X-线微区分析术检测,未见La、Sm及Yb的峰值,显示了无细胞内的累积现象;SCE检测,小剂量LaCl_3长期作用后,未见姐妹染色单体交换增多。
This paper reports the long term effects of small dosage of LaC13, SmCl3 and YbCl3 on cultured cells by autoradiography, electron microscopy, x-ray microanalysis and SCE technique. The results showed that human breast cancer cells, when exposed respectively to 0. 1mmol/L of LaCl3, SmCl3 and YbCl3 for 16 months, would show increased mitotic activity and synthesis of DNA, while the surface structures and ultradtructure of the cells displayed no changes. X-ray micro-analysis did not reveal intracellular deposition of La, Sm and Yb. SCE technique demonstrated no change in the genetic material.
出处
《白求恩医科大学学报》
CSCD
1992年第6期515-517,T001,共4页
Journal of Norman Bethune University of Medical Science
基金
国家自然科学基金
关键词
镧
钐
镱
生物作用
细胞培养
Lanthanum Samarium Ytterbium Cell culture X-ray microanalysis SCE
