吡咯烷酮酶试验在沙门菌鉴定中的应用

Application of PYR test in identification of salmonella

〔目的〕探讨吡咯烷酮酶 (PYR)试验在鉴定沙门菌中的价值。〔方法〕将实验菌株作常规生化反应 ,血清学凝集 , 噬菌体裂解等试验进行鉴定 ,并同时做PYR试验 ,观察其结果。〔结果〕14 8株沙门菌全部PYR试验阴性 ,3 2株枸橼酸杆菌全部为阳性。〔结论〕PYR试验可作为鉴别沙门菌与枸橼酸杆菌一种有价值的试验 ,对其它肠杆菌科细菌也有一定的鉴别意义。

Objective To investigate the value of PYR test in identification of salmonella.〔Methods〕 A total of 148 strains of salmonella and 32 strains of citrobacter were obtained using microbiological reaction,serum identification and bacteriophage crack test.PYR test was conducted for each strain.〔Results〕 All the strains of salmonella were negative while all those of citrobacter positive for PYR test.〔Conclusions〕PYR test can be used to differentiate salmonella from citrobacter.