中药消痰散结方抑制裸鼠原位移植人胃癌SGC-7901的生长转移

Xiaotansanjie recipe inhibits growth and metastasis of human gastric adenocarcinoma cell SGC-7901 transplanted in nude mouse

目的:研究中药消痰散结方对裸鼠原位移植人胃癌生长转移的抑制作用. 方法:建立裸鼠原位移植人胃癌模型;实验分组:对照组、中药组、化疗组、联合组各10只;观察和比较各组肿瘤抑制率和肿瘤转移情况.用免疫组化法定性分析和比较肿瘤转移相关指标P21 ras,P185,VEGF蛋白,KDR蛋白的表达,用RT-PCR法定量分析和比较各组ras,cerbB2, vEGF,KDR的表达. 结果:中药组、化疗组和联合组的抑瘤率分别为72.0%、51.3%、70.1%;在远处淋巴结转移率和脏器转移率方面, 与对照组相比,中药组、化疗组和联合组均减少了转移的发生,有显著性差异(P<0.05).中药组、化疗组、联合组在不同程度上下调了ras,cerbB2,VEGF和KDR在蛋白水平和mRNA水平的表达. 结论:消痰散结方具有抑制裸鼠原位移植入胃癌生长和转移的作用,其作用机制可能与下调了肿瘤转移相关基因ras, cerbB2,VEGF,KDR的表达有关.

AIM: To investigate the effects of the Xiaotansanjie recipe on the nude mouse model of human gastric carcinoma cells using orthotopic transplantation. METHODS: Firstly we established the metastatic gastric carcinoma model of nude mice by orthotopic implantation. On the second day they were devided into four groups at random. The inhibitary rates of tumour growth were detected, and local invasiveness, the rates of metastasis in the local lymph node, lung, liver and peritoneum were compared between different groups. By Envision immunohistochemical method and semiquantitative reverse transcription poly-merase chain reation, the expression of P21ras, P185, Ras, CerbB2, VEGF and KDR in gastric carcinoma were determined. RESULTS: The inhibitary rates of Xiaotansanjie recipe group, 5-Fu group and combined group were 72.0% , 51.3% and 70.1% respectively. Compared with the control group, the rates of local invasiveness, metastasis in the lymph node and distal organs in other three groups were significantly lower (P<0.05). The positive rate of P21ras, P185, Ras, CerbB2, VEGF and KDR in Xiaotansanjie recipe group obviously lower than those in control group (P<0.05). CONCLUSION: Xiao Recipe has a strong inhibitory effect on both growth and metastasis of gastric cancer, and the mechanism of that may related with the reduced expression of oncogene cerbB2, ras,VEGF and KDR.