吲哚美辛和、或顺铂对胃癌细胞凋亡的影响

Effects of indomethacin and/or casplatin on apoptosis of gastric cancer cells

目的:探讨吲哚美辛(indomethacin,IN)、顺铂(cisplatin, CDDP)对胃癌细胞株MGC803凋亡的影响,为寻求新的胃癌治疗策略提供理论依据. 方法:MGC803细胞经IN、CDDP处理后,用MTT法检测药物对细胞增生的抑制;荧光染色检测细胞凋亡率;流式细胞仪测定细胞周期. 结果:IN、CDDP均可抑制MGC803细胞的增生、并诱导其凋亡;IN对胃癌细胞系MGC803的作用有较强的量效和时效依赖关系;高浓度的CDDP(10 mg/L)对此胃癌细胞系有较强的时效依赖关系,而低浓度的CDDP(0.1 mg/L, 1 mg/L)在作用24 h后,由于肿瘤细胞的耐药机制,细胞凋亡率不再发生改变;中等剂量IN(200 umoL/L)加各种浓度CDDP对此胃癌细胞系也有较强的量效和时效依赖关系,且加IN后,低浓度的顺铂诱导凋亡作用接近于单独作用组高浓度顺铂的效用. 结论:吲哚美辛与顺铂对胃癌细胞株MGC803有协同作用,IN可能能够对抗胃癌对化疗药物的耐药.

AIM: To investigate the effects of indomethacin (IN) and casplain (CDDP) on apoptosis of gastric cancer cell line MGC803 and to provide the theoretic basis for gastric cancer therapy. METHODS: Gastric cancer MGC803 cells were treated with IN and/or CDDP. Proliferation of the cells was detected by using MTT assay. Apoptosis of cells was measured by using fluorescence staining, and cell cycle kinetics by flow cytometry. RESULTS: Both IN and CDDP were able to restrain the proliferation and induce apoptosis of the cells. There was dose-dependent and time-dependent cell proliferation induced by IN. High concentration of CDDP (10 mg/L) had also the time-effect, but a low dose of CDDP (0.1, 1 mg/L) did not. The percentage of apoptotic cells did not changed after 24h incubation with a low dose of CDDP. There also existed good dose-dependent and time-dependent effects when every concentration of CDDP combined with moderate dose IN (200 μmol/L). Low dose CDDP with IN had the similar effect to that of high dose CDDP alone. CONCLUSION: IN and CDDP have the synergistic action when they work together on gastric cancer cell line MGC-803 and perhaps IN can antagonize the chemo-resistance of gastric cancer to other chemical drugs.