摘要
目的 探讨血管紧张素转化酶(ACE)抑制剂对心房颤动(房颤)心房组织细胞外信号调节激酶的影响。方法 52例风湿性心脏病患者分成四组,窦性心律组、窦性心律+卡托普利组、慢性房颤组(房颤>6个月)、慢性房颤+卡托普利组。外科手术时取右心耳组织,采用逆转录-聚合酶链反应测定细胞外信号调节激酶(ERK)mRNA量,免疫印迹法(Western blotting)测定激活ERK蛋白质量。结果 ERK_2 mRNA量在窦性心律组和窦性心律+卡托普利组明显低于慢性房颤组和慢性房颤+卡托普利组(P<0.01),窦性心律组和窦性心律+卡托普利组比较、慢性房颤组和慢性房颤+卡托普利组比较差异无显著性(P>0.05);激活ERK_1/ERK_2量在慢性房颤+卡托普利组明显低于慢性房颤组(P<0.01),但明显高于窦性心律组和窦性心律+卡托普利组(P<0.01)。结论 ACE抑制剂可部分阻断房颤时心房组织内“致纤维化”信号转导通路的激活,对心房结构重构产生一定的有益的影响。
Objective To investigate the effect of angiotensin-converting enzyme (ACE) inhibitor on extracellular signal-regulated kinase(ERK) of atrial tissue during atrial fibrillation(AF). Methods Fifty-two patients with rheumatic heart disease were divided into 4 groups: sinus rhythm (SR) group, SR plus catopril group, chronic AF group(AF>6 months), and chronic AF plus catopril group. Atrial tissue was obtained from right atrial appendage during open heart surgery. The mRNA amounts of ERK were measured by quantitative reverse transcription polymerase chain reaction(RT-PCR). The protein amounts of activated ERK_1/ERK_2 were measured by Western blotting. Results The mRNA amount of ERK_2 of both SR group and SR plus catopril group were significantly lower than those of both chronic AF group and chronic AF plus catopril group(P<0.01). The mRNA amount of ERK_2 of SR group was similar with that of SR plus catopril group, and so was chronic AF group with chronic AF plus catopril group(P>0.05). The protein amounts of activated ERK_1/ERK_2 of chronic AF plus catopril group were significantly lower than those of chronic AF group, but higher than those of both SR group and SR plus catopril group (P<0.01). Conclusion ACE inhibitor can partly block 'profibrotic' signaling pathway during AF. It may has beneficial effect on atrial structural remodeling of fibrillating atria.
出处
《中华心律失常学杂志》
2004年第2期109-112,共4页
Chinese Journal of Cardiac Arrhythmias
