里氏木霉306生物合成t-PA的代谢调节机制的研究

Study on Metabolic Control Mechanism of tPA (Tissue-Type Plasminogen Activator)Production by Recombinant Strain of Trichoderma reesei 306

对基因工程菌株里氏木霉 (Trichodermareesei) 30 6生物合成组织型纤溶酶原激活剂 (t PA)的代谢调节机制进行了研究。在基础发酵条件下 ,L 山梨糖、D 果糖、可溶性纤维素、CMC、麦芽糖、乳糖和棉子糖等单糖及多糖对t PA生物合成都有诱导作用 ,其中L 山梨糖的诱导效果最好 ,加量以 1 .0 %为宜。葡萄糖及其中间代谢产物对t PA的生物合成产生分解代谢物阻遏作用。纤维二糖在低浓度时可以促进t PA的生物合成而在高浓度时对t

Metabolic control mechanism of tissuetype plasminogen activator (tPA) produced by recombinant strain of Trichoderma reesei 306 was studied. Under the minimal fermentation conditions, monosaccharides and polysaccharides such as sucrose, carboxymethyl cellulose, raffinose, Lsorbose, lactose, dissoluble cellulose and maltose play induction role in the biosynthesis of tPA. Among them, Lsorbose has the best induction result at the concentration of 1.0%.While glucose and its intermediate metabolites play repressing role on tPA biosynthesis by producing catabolites. And low concentration of cellobiose could promote biosynthesis of tPA, however, high concentration of it play feedback repression role.;