石竹细胞悬浮培养研究(英文)

Study on cell suspension culture of Dianthus chinensis

石竹细胞继代周期为 7d时 ,悬浮细胞培养系生长最快 ,生长率最高 ,而且培养物中胚性细胞较多 ,并能保持较快的分裂和生长 ,能促进已形成的大细胞团的生长和分化。转代时接种物与新鲜培养基的体积比以1∶2较好 ,悬浮系细胞生长最快 ,生长率最高 ,以 1∶2和 1∶3的高倍稀释接种有利于胚性细胞的形成及产生小的胚性细胞团 ,对悬浮系添加椰乳和水解乳蛋白的混合物 ,可较大幅度地提高悬浮细胞系的生长速率 ,单独添加上述两种物质的效果均不如二者的综合效应好。在 6种不同激素组合中 ,配方 2 (2 ,4 D 1 .5mg/L +NAA0 .5mg/L +6 BA 0 .5mg/L)最好 ,生长率最高。配方 5 (2 ,4 D 1 .5mg/L +NAA 0 .5mg/L +6 BA 1 .0mg/L)其次 ;配方 1 (2 ,4 D 1 .0mg/L +NAA 0 .5mg/L +6 BA 0 .5mg/L)次之。

In the present research,the suspension culture clones of Dianthus chinensis can keep cell multiplication and growth-rate at high speed when the secondary cycle is arranged for 7 days. Moreover,there exist lots of embryonic cells in the culture fluid that could keep growth and division fast and promote growth and differentiation of the formed big cell groups. When conducting trans-generation,it is better if volume rate between inocula and fresh culture media is 1∶2. Because the cell multiplication and cells~, growth keep the fastest under this condition. It is the high dilution rate of 1∶2 or 1∶3 that will contribute to form the embryonic cells and produce the small embryonic cell groups. Provided that a mixture of coconut milk(CK)and lacotalbumin hydrolysate(LH)is added,the growth-rate of suspension clones could be greatly improved. Adding both is better than adding only one of them. The concentration of hormone plays a key role in cells~, growth and division. Compared to six different formulas,formula 2(2,4-D 1.5 mg/L+NAA 0.5 mg/L+6-BA 0.5 mg/L)is the best. Formula 5 comes second (2,4-D 1.5 mg/L+NAA 0.5 mg/L+6-BA 1.0 mg/L)and formula 1(2,4-D 1.0 mg/L+NAA 0.5 mg/L+6-BA 0.5 mg/L)comes third.