植物生长调节剂BR和SHK-6对大豆生物产量和根瘤固氮活性的激素调控研究1

HORMONAL REGULATION OF PLANT GROWTH REGULATOR BR AND SHK-6 ON SOYBEAN BIOMASS AND NITROGENASE ACTIVITY

应用快生根瘤菌 (Sinorhizobiumxinjiangensis ,CCBAU 1 1 0 T)和慢生根瘤菌 (Bradyrhizobiumjaponicum T,USDA6 T)接种大豆 ,在无菌沙培条件下叶面喷施植物生长调节剂BR和SHK - 6研究调节剂对大豆植株及根瘤固氮的激素调控效应。结果表明 :1 )不同根瘤菌接种条件下 ,BR和SHK - 6处理大豆根干重和茎干重得到显著增加 ,提高了根瘤数、根瘤重和根瘤固氮活性。 2 )BR和SHK - 6处理提高了根瘤固氮能力 ,根瘤、根和茎的含氮量得到明显提高。 3)BR和SHK - 6处理通过改变激素平衡来调控根瘤固氮 ,其中CTK/GA、CTK/IAA对根瘤固氮有正调控作用 。

A comparative study was conducted on regulation of brassinolide(BR) and SHK-6 on the symbiosis between soybean and rhizobium strains of CCBAU110 T and USDA6 T under sterility sandy culture and pot experiment. The results showed that BR and SHK-6 significantly increased the dry weight of root and shoot, nodule numbers nodule weight and nitrogenase activity in different inoculated rhizobium strains. The nitrogen content was evidently increased by BR and SHK-6 in the nodule ,root and shoots. Plant growth regulator regulated the nodule number and fixing nitrogen activity by the hormone balance. It was found that the CTK/GA and CTK/IAA was up-regulation of nodule fixing nitrogen activity, and the IAA/ABA and GA/ABA was down-regulation of nodule fixing nitrogen activity.