胃异型增生和早期胃癌AgNOR图像定量分析和PCNA表达

AgNOR image quantitative analysis and PCNA expression in gastric dysplasia and early stage gastric cancer

目的 :探讨区嗜银蛋白 (AgNOR)定量分析和增殖细胞核抗原 (PCNA)表达在胃黏膜异型增生和早期胃癌中的意义。方法 :对 5 0例胃镜活组织检查标本的胃黏膜异型增生、早期胃癌的区AgNOR经图像分析系统进行定量分析和PCNA免疫组织化学染色。结果 :正常胃黏膜和中高度异型增生之间AgNOR/细胞核与PCNA增殖指数差异均有显著性 (P <0 .0 1 ) ;中～高度异型增生和早期胃癌之间AgNOR/细胞核和PCNA增殖指数差异均有显著性 (P <0 .0 1 )。 结论 :胃黏膜中高度异型增生。

Objective:To investigate the significance of the argyrophilie nucleolar organizer regions associated protein(AgNOR) image quantitative analysis and PCNA expression in gastric dysplasia and early stage gastric cancer.Methods:Fifty cases of biopsy specimen with gastric mucosal dysplasia and AgNOR were analysed quantitatively by employing image analysis and the expression of PCNA with immunohistochemical staining.Results:There was significant difference between normal gastric muscosa,moderate or severe dysplasia of the AgNOR/cell nucleolar and PCNA expression( P <0.01).The results also suggested that there was significant difference between moderate or severe dysplasia,the AgNOR/cell nuclear of early stage gastric cancer and PCNA expression rate ( P <0.01).Conclusions:The gastric mucosa of moderate or severe dysplasia,AgNOR/cell nuclear of early stage gastric cancer and PCNA expression rate present positive correlation.