肺癌患者红细胞CD35、CD44s、CD58测定及其意义

Significance of quantitative assay of CD35?CD44s and CD58 expressed on erythrocytes in patients with lung cancer

目的 :探讨肺癌患者红细胞CD35、CD4 4s、CD5 8在不同病理类型、不同分期及化疗前后分子的数量变化。方法 :参照王海滨方法 ,将经过处理的定量红细胞悬液 ,移入V型板中 ,依次加入CD35、CD4 4s、CD5 8单克隆抗体。进行细胞酶免疫分析。取上清液 ,在酶标仪 4 0 5nm读取其吸光度A值 ;减去对照孔A值为标本的的测定值。结果 :不同病理类型的肺癌红细胞CD35、CD4 4s和CD5 8除差分化癌外 ,均较正常组降低 (P <0 .0 5～P <0 .0 1 )。但各病理类型之间红细胞CD35、CD4 4s、CD5 8比较 ,差异均无显著性 (P >0 .0 5 )。Ⅲ、Ⅳ期肺癌组红细胞CD35和CD4 4s较Ⅰ、Ⅱ期肺癌组降低 (P <0 .0 5 )。化疗组肺癌红细胞CD35较非化疗组降低 (P <0 .0 5 ) ,CD4 4s和CD5 8两组比较 ,差异均无显著性 (P >0 .0 5 )。结论 :肺癌患者红细胞CD35、CD4 4s、CD5 8较正常人降低 ,且随着病情的进展红细胞免疫功能进一步下降 ,表明红细胞CD35、CD4 4s、CD5 8分子数量的变化与肿瘤的转移和恶化程度有关。这种关系的紊乱可能对肺癌的发生、发展。

Objective:To investigate the relationship between quantitative molecule expresssion of CD35,CD44s and CD58 on erythrocytes and types of pathology,stages and before and after chemical treatment in lung cancer.Methods:Glutaraldehyde fixed red blood cells were analyzed in 'V' microtitre plates,mouse monoclonal anti human CD35,CD44s and CD58 were added in order.After immunoenzymic reaction process in the plates,the red bloodcell supernatant was transferred to a clean microtitre plate and read at 405nm (A405).Results:Except poorly differentiated lung cancer,CD35,CD44s and CD58 expressed were significantly lower on erythrocytes in various pathologic types in lung cancer groups than in control group.The difference was significant( P <0.05 and P <0.01).The difference was not significant in CD35,CD44s and CD58 expressed( P <0.05) on erythrocytes in various pathologic types of lung cancer ( P >0.05).It showed that quantitative molecule expression of CD35,CD44s and CD58 in the patients with lung cancer in Ⅲ and Ⅳ stages were significantly lower than that in Ⅰ and Ⅱ stages( P <0.05).Compared with the patients with lung cancer before chemical treatment,expression of CD35 was significantly lower in the patients with lung cancer after chemical treatment.There was no significant difference between CD44s and CD58 molecule expressions( P >0.05).Conclusions:Our data suggest that quantitative molecule expression of CD35,CD44s and CD58 on erythrocytes in the patients with lung cancer is lower than normal and the erythrocytes immuno function descends with the aggravation of the disease.Furthermore,this data displays that change of CD35,CD44s and CD58 quantities expression on erythrocytes is associated with metastasis and deterioration of the tumor in the patients with lung cancer,which may be of importantce to the development and prognosis of lung cancer.