抑制氯通道阻抑鼻咽癌细胞周期和细胞增殖

Inhibition of cell proliferation and arrest of cell cycle progression by blocking Cl- channels in nasopharyngeal carcinoma cells

目的 :研究Cl-通道在鼻咽癌细胞调节性容积回缩 (RVD)、增殖及细胞周期分布中的作用。方法 :活细胞图像分析低分化鼻咽癌细胞 (CNE - 2Z)RVD ,用台盼蓝拒染法检测细胞存活率 ,MTT法检测细胞增殖能力。用流式细胞仪测定细胞周期不同时相细胞百分率。结果 :Cl-通道抑制剂硝基苯丙胺基苯甲酸 (NPPB)剂量依赖性抑制RVD和细胞增殖 ,10 0 μmol/LNPPB明显阻抑细胞周期进程 ,使细胞停滞于G1期 ,G1期细胞百分率从 5 4 %提高到71% ,但对细胞存活率没有显著影响。结论 :阻抑Cl-通道可阻滞细胞于G1期而抑制细胞增殖。提示Cl-通道和RVD的激活是促进细胞从G1期进入S期和维持增殖所必需的因素。

AIM: The roles of Cl-channels in regulatory volume decrease (RVD), cell proliferation and cell cycle progression in nasopharyngeal carcinoma cells (CNE-2Z) were investigated. METHODS: Image analysis of living cells was used to detect the volume changes following exposure to hypotonic solutions. Cell viability was determined by the trypan blue assay. MTT method was applied to detected cell proliferation. The effect of the blocker on the cell cycle distribution was monitored by the flow cytometry. RESULTS: 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) inhibited RVD and cell proliferation in a dose-dependent manner. NPPB at the concentration of 100 μmol/L arrested cells in G 1 phase (G 1 population increased from 54% to 71% at 48 h after treatments), but did not significantly alter cell viability. CONCLUSION: Block of chloride channels suppressed cell proliferation by arresting cells in G 1 phase. The results suggest that activation of Cl-channels and RVD is necessary for facilitating cells to proceed to the S phase from G 1 phase and maintaining cell proliferation.