星形胶质细胞正常条件培养液对体外脑缺氧/复氧损伤神经元的影响

Influence of normal conditioned medium of astrocytes on the damaged neurons of cerebral hypoxia and reoxygenation in vitro

目的 :探讨星形胶质细胞正常条件培养液 (NCMA)对体外脑缺氧 /复氧 (H/R)损伤神经元的营养性作用。方法 :先行大鼠大脑皮质星形胶质细胞和神经元的分离纯化培养 ,然后用NCMA以不同的浓度培养正常及脑H/R损伤的神经元 ,通过MTT法测定其神经元的活性和存活率。结果 :NCMA能提高正常及脑H/R损伤神经元的活性和存活率。结论 :NCMA对培养的神经元具有营养性支持作用。

AIM: To study the nutritional role of the normal conditioned medium of astrocytes (NCMA) on the injured cerebral cortex neurons induced by cerebral hypoxia and reoxygenation in vitro. METHODS: The normal and damaged neurons induced by hypoxia and reoxygenation were cultured with normal conditioned medium of astrocytes (NCMA) after the cerebral cortical astrocytes and neurons of rats were isolated. The viability and survival rate of cultured neurons were investigated by MTT method. RESULTS: NCMA increased the viability and survival rate of cultured neurons. CONCLUSION: NCMA has nutritional and supporting roles on cultured neurons.