补肾宁心方对人单核-血管内皮细胞粘附的影响

Effects of the Bushen Ningxin Chinese herb-containing serum on the adherence of human monocytes to endothelial cells

目的 :探讨补肾宁心方对人单核 -血管内皮细胞粘附的影响及机理。方法 :以培养人脐静脉内皮细胞 (HUVECs)作为靶细胞 ,在内皮细胞培养基中加入氧化的低密度脂蛋白 (ox -LDL)或在试验体系中加入灌服补肾宁心方的兔血清 ,以孟加拉玫瑰红活细胞染色法测定人单核细胞系U937与HUVECs的粘附 ,并用流式细胞仪检测内皮细胞表面粘附分子细胞间粘附分子 - 1(ICAM - 1)、血管细胞粘附分子 - 1(VCAM - 1)以及E -选择素的表达。结果 :ox -LDL显著增强单核U937细胞与内皮细胞之间的相互粘附 ,如在试验体系中加入灌服补肾宁心方的动物血清 ,则粘附率明显降低 (P <0 0 1)。流式细胞仪分析结果显示ox -LDL能明显促进内皮细胞表面ICAM - 1、VCAM -1以及E -选择素的表达 ,补肾宁心方中药灌服血清可显著下调内皮细胞表面ICAM - 1、VCAM - 1以及E -选择素的表达 (P <0 0 1)。结论 :补肾宁心方含药血清可能通过下调内皮细胞表面粘附分子的表达抑制单核 -血管内皮细胞粘附 ,从而发挥对血管内皮细胞的保护作用。

AIM: To study effect of the Bushen Ningxin decoction, a Chinese medicine, on the adherence of monocytes to endothelial cells and its mechanism. METHODS: Using cultured human umbilical vein endothelial cells (HUVECs) as target cells, oxidized low density lipoprotein (ox-LDL) was added to the endothelial cell culture to prepare the model of human endothelial cell injury. The serum of rabbits having been treated with Bushen Ningxin decoction was added to trial architecture, the adherence of monocyte-like cell line U937 to HUVECs was analyzed using Rose Bengal staining. In addition, the expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1(VCAM-1) and E-selectin in HUVECs was measured by flow cytometry. RESULTS: Treatment of HUVEC with ox-LDL (100 mg/L) for 24 hours significantly increased adhesion of U937 to HUVECs. If serum of the animal treated with Bushen Ningxin decoction was added to trial architecture, the adhesion decreased significantly. The flow cytometry analysis showed that ox-LDL could induce the expression of ICAM-1, VCAM-1 and E-selectin in HUVECs. Serum of the animal treated with Bushen Ningxin decoction significantly decreased the expression of ICAM-1, VCAM-1 and E-selectin in HUVECs. CONCLUSION: The Bushen Ningxin Chinese herb-containing serum has an inhibitory effect on the adherence of monocytes to endothelial cells, probably by way of down-regulating the expression of ICAM-1, VCAM-1 and E -selectin in endothelial cells.