摘要
目的 :研究酿酒酵母细胞表面展示的人衰变加速因子 (hDAF) ,是否具有天然衰变加速因子的补体抑制活性。方法 :先用正常人血清处理酵母细胞EBY10 0 ,然后用流式细胞仪检测酵母细胞表面C5b 9复合物的沉积和hDAF在酵母细胞表面的表达。结果 :酵母细胞EBY10 0可直接活化人血清中的补体成分 ,导致酵母细胞表面C5b 9的沉积 ;而表达DAF的酵母细胞表面C5b 9的沉积明显减少。结论 :酵母细胞表面展示的DAF可正确折叠 。
AIM: To investigate whether the hDAF displayed on the surface of yeast cells can inhibit the deposition of C5b 9. METHODS: After treatment with normal human serum, deposition of human complement C5b 9 and DAF expression on yeast cells EBY100 were analyzed by flow cytometry. RESULTS: Yeast cells EBY100 could activate the complement molecules in human serum and lead to the deposition of C5b 9, while the deposition of C5b 9 on the surface of yeast cells with hDAF could significantly reduce the deposition of C5b 9. CONCLUSION: hDAF displayed on the surface of yeast cells can fold correctly and exhibit activity of complement’s inhibition.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2004年第3期269-271,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目 (No .30 0 80 0 32
30 1 2 80 1 9)
关键词
补体
衰变加速因子
酵母细胞
表面展示
complement
decay accelerating factor
yeast cell
surface display
