TLSF_(JM)对Th1/Th2样细胞亚群比率的影响

The influnce of TLSF_(JM) on the proportion of Th1/Th2-like cell subsets

目的 :探讨TLSFJM 对同种异体抗原活化的Th1/Th2样细胞亚群变化的影响。方法 :在混合淋巴细胞反应 (MLR)体系中加入TLSFJM 或IL 4 ,用细胞内免疫荧光染色结合流式细胞术分析TLSFJM 对Th1/Th2样细胞亚群比率的影响。结果 :在TLSFJM实验组中 ,活化淋巴母细胞分化为IFN γ+ 细胞的比率略有降低 (49.8%→ 4 3.1% ) ,IL 4 + 、IL 6 + 细胞的比率有明显降低 ,IL 4 + 细胞的比率由 75 .4 %降至 4 3.7% ,IL 6 + 细胞的比率由6 7.8%降至 5 2 .6 %。在未活化小淋巴细胞中 ,也观察到同样的趋势。结论 :TLSFJM 对Th1、Th2样细胞亚群都有抑制 ,但似乎主要作用于Th2样细胞亚群 。

AIM: To explore the influence of TLSFJM on the proportion of alloantigen activated Th1 and Th2 like cell subsets. METHODS: TLSFJM or IL 4 was added to mixed lymocyte reaction(MLR) system. The influnce of TLSFJM on the proportion of Th1 and Th2 like cell subsets was analyzed by intracellular immunofluorescence staining and FACS. RESULTS: In the TLSFJM group, the proportion of IFN γ + cells differentiated from activated lymphoblast descended from 49.8% to 43.1%, IL 4 + cells from 75.4% to 43.7% and IL 6 + cells from 67.8% to 52.6%. The similar tendency was also observed in the unactivated small lymphocytes. CONCLUSION: TLSFJM can inhibit both the Th1 and Th2 like cell subsets, but mainly inhibit the Th2 like cell subset, thereby reducing the proportion of Th2 like cell subsets.