加味保元汤注射液抗心律失常的实验研究

Study on antiarrhythmia effects of Baoyuantang injection

应用中药复方加味保元汤注射液 (BYI)对快、慢两型多种心律失常动物模型进行了实验研究 ,目的 :观察复方中药制剂加味保元汤抗实验性心律失常的疗效。方法 :72只新西兰种家兔随机分为实验组与阳性对照组和阴性对照组 ,前者静注BYI10 0～ 15 0mg/kg ,后者分别静注盐酸利多卡因 5mg/kg(第四组阳性对照应用异丙肾上腺素 0 .2 5mg/kg)和生理盐水 (NS) 10 0～ 15 0mg/kg。各组再分为 4组 ,于注射BYI及盐酸利多卡因 ,NS5min后分别静脉给予 :肾上腺素 5 0 μg/kg ,5 0 0mg/L的乌头碱 0 .8ml/min灌流 ,5 %Ca Cl2 2mg/min灌流及异搏定 1mg/kg。用上述药物分别诱发严重心律失常 ,观察BYI对实验组心律失常发生率、发生时间、持续时间及死亡率等指标的影响 ,并与对照组进行比较。结果 :保元汤注射液能显著推迟并缩短由肾上腺素诱发的家兔心律失常时间 ;可显著降低由乌头碱所致的心室颤动 (VF)发生率和死亡率 ;可显著降低CaCl2 所致的VF发生率 ,明显延长动物的存活时间 ;可显著推迟并缩短由异搏定诱发的缓慢型心律失常 ,降低Ⅱ、Ⅲ度房室传导阻滞的发生率。结果 :加味保元汤注射液可延缓、降低甚至阻止严重心律失常的发生 ,对心律失常具有良好的共轭性屏障治疗作用。

Objective: To observes the effects of chinese herbal medicine of Baoyuantang injection(BYI,保元汤注射液) on antiarrhythmia. Methods: 72 rabbits were divided into three parts. The part for test was injected in vein BYI100-150mg/kg (the treatment group), the parts for contrastion were injected in vein Lidocaine hydrochloride injection 5mg/kg, (the positive control group) (The fourth group use IPA) and normol saline 100-150mg/kg. (the negative control group). each part was devided into four subgroups, and after injecting the BYI, Lidocaine hydrochloride injection or normal saline five minutes, every subgroup was injected in vein these medicine: adrenal in 50μg/kg; aconitine 500mg/L, 0.8ml/min; 5%CaCl-2 2ml/min; verapamililmg/kg. These medicines cause the serious cardiac arrhythmias partly, and we observe the effects of BYI about the incidence rate on cardiac arrhythmias, the occurring time, the lasting time and the mortality ect, and compare to the two parts for contrastion. Results: The experiments showed that BYI could remarkable delay and shorten cardiac arrhythmias induced by adrenalin, reduced occurring rate and mortality of ventricular fibrillation induced by a conitine and ouabain; decreased incidence rate of CaCl-2 induced ventricular fibrillation; and evistent time of animal wrer prolonger. The slow type of cardiac arrhythmias induced by verapamili was delayed and reduced, and occurring rate of a trioventricular block(AVB) of three grade was decreased. Conclusions: BYI may delay, reduce or even prevent occurrence serious cardiac arrhythmias. It has a satisfactory two way barrier clinical effect on cardiac arrhythmias.