摘要
【目的】比较 3种方法检测产超广谱β -内酰胺酶 (ESBLs)菌株的检出率 ,了解近期广东省人民医院大肠埃希菌和肺炎克雷伯菌产ESBLs菌株的感染率及其耐药情况。【方法】walkaway - 4 0机鉴定菌种 ,MIC初筛 ,双纸片协同试验 ,确证试验、药敏纸片扩散法。【结果】大肠埃希菌 72株和肺炎克雷伯菌 78株 ,确证试验检出率分别为 4 6 %和 33%。上述产ESBLs菌株对三代头孢菌素和氨曲南大多耐药 ,对氨苄西林 ,环丙沙星多呈耐药 ,亚胺培南 10 0 %敏感 ,对 5种复方制剂耐药率为 18 2 %~ 10 0 %。【结论】双纸片协同法和NCCLS确证试验均是检测ESBLs的好方法 ,各实验室应重视ESBLs菌株的检测和药敏试验。
To compare the detection rate of three methods for detection of extended-specturm β-lactamases(ESBLs)-producing strains and to study the injecting rate and drug-resistant condition of ESBLs-producing E.coli and Klebsiella pneumomiae at the GuangDong Province′s Hospital.(Walkaway-40 type automatic bacterial identification/drug susceptibility analyzer)MIC Primary screening test.Double paper synergistic test.NCCLS validation test,K-B drug susceptibility paper diffusion test.The results showed that among 72 strains of E.Coli and 78 strains of Klebsiella pneumomiae,there were 46% and 33% detection rate detected by double paper synergistic test and NCCLS vali dationtest.Among ESBLs positive strains of E.Coli and Klebsiella pneumomiae,Most of them showed drug-resistant to cephal or sporins Ⅲ,Aztreonam,Ampicillin and Ciprofloxacin.most of them showed sensitive(100%) to imipenem and drug-resistant(18.2%~100%) to antibiotic-CO.[Conclusion]Double paper synergistic test and NCCLS vali dation test are very nice methods for detecting ESBLs.Each laboratory should appreciate the detection of ESBLs strains and drug susceptibility test.
出处
《武警医学院学报》
CAS
2004年第3期196-198,共3页
Acta Academiae Medicinae CPAPF
关键词
超广谱Β-内酰胺酶
大肠埃希菌
肺炎克雷伯菌
Extended-spectrum
β-lactamases(ESBLs)
Escherichia coli
Klebsiella pneumomiae