灵芪菌质发酵工艺初报

A preliminary report on solid-state fermentation of Ganoderma lucidum with Radix Astragali containing medium

目的 :探讨灵芪菌质发酵的工艺 ,验证药用真菌新型固体发酵的优越性。方法 :将灵芝分别在普通基质、药性基质 (含黄芪 )、富硒药性基质中发酵 ,对三种基质中菌质的有效部位多糖的含量进行测定及动态研究 ,比较其变化。结果 :三种基质中菌质有效部位多糖含量及其相对标准差分别为 4 .6 5 %、1.6 1% ,3.76 %、1.99% ,4 .5 0 %、1.86 % ;对灵芝和黄芪发酵组合的有效成分多糖、蛋白质及总皂苷进行动态变化研究 ,发现第 2 8天是发生次生代谢最旺盛的时候 ,确定为发酵终点。结论 :灵芝和黄芪发酵组合是可行的。

Objective: To test the practicability of the solid-state fermentation for medicinal fungi by fermenting Ganoderma lucidum with Radix Astragali containing medium. Methods: Ganoderma lucidum was fermented in ordinary medium, drug-containing medium (containing Radix Astragali) and selenium-rich drug-containing medium respectively. The polysaccharide contents of fermentation products from the three kinds of culture media were tested at different time, and the changes were compared. Results:The polysaccharide contents of fermentation products from the three kinds of culture media were 4.65%, 3.76% and 4.50% respectively and their relative standard deviation were 1.61%, 1.99% and 1.86% respectively. By observing the changes of the contents of polysaccharide, protein and total saponin in fermentation products from the drug-containing medium at different time, it was found that the 28 th fermentation day was the time when secondary metabolism was most active, and it should be the fermented terminal point.Conclusion:The fermentative combination of Ganoderma lucidum and Radix Astragali is practicable.