益气活血方药对衰老大鼠肝脏细胞增殖和凋亡的影响

Effect of recipes replenishing qi and activating blood on cell proliferation and apoptosis in the liver of aging rats

目的 :了解益气活血方药对自然衰老大鼠肝脏组织细胞增殖和凋亡的影响。方法 :利用自然衰老大鼠模型 ,连续给药 4个月 ,观察益气和活血方药对模型动物肝脏组织细胞增殖和凋亡衰老性变化的改善作用。结果 :(1)与年轻大鼠比较 ,老年大鼠肝脏组织的G0 G1期细胞比例增加 (P <0 .0 1) ,而S期细胞及G2 M期细胞比例减少 (P <0 .0 1) ;同时凋亡细胞的比例增加 (P <0 .0 1)。 (2 )与老年大鼠空白组比较 ,益气组和活血组动物肝脏组织的G0 G1期细胞比例减少 (P <0 .0 1) ,活血组比益气组减少更明显 (P <0 .0 1) ;S期和G2 M期细胞比例增加 (P <0 .0 1) ,益气组和活血组比较差异不显著 (P >0 .0 5 )。 (3)益气和活血方药皆能减少老年大鼠肝脏组织细胞的凋亡 (P <0 .0 1) ,但益气方药的作用明显优于活血方药 (P <0 .0 1)。结论 :(1)自然衰老大鼠肝组织细胞增殖减少而细胞凋亡增加 ;(2 )益气和活血方药均能促进衰老大鼠肝脏组织的细胞增殖 ,活血方药作用稍强 ;(3)益气和活血方药均能抑制自然衰老大鼠肝组织细胞凋亡 ,益气方药作用略优。

Objective:To observe the effect of recipes replenishing qi and activating blood on cell proliferation and apoptosis in the liver of natural aging rats. Methods:Natural aging rats were under administration of recipes replenishing qi or activating blood for 4 months. The liver of the rats was prepared into cell suspension for determination of cell proliferation and apoptosis with PI-staining and flow cytometer.Results:(1)Compared with those of the young rats,the cells in G 0-G 1 phase in the liver tissue of aging rats were increased(P<0.01),and apoptosis cells were increased(P<0.01), while the cells in S and G 2-M phases were decreased(P<0.01). (2)Compared with those of the aging rats,the cells in G 0-G 1 phase in the liver tissue of aging rats administered recipes replenishing qi or activating blood were decreased(P<0.01),and it was more obvious in activating blood group than in replenishing qi group(P<0.01); the cells in S and G 2-M phases were increased(P<0.01),and there was no significant difference between the activating blood group and the replenishing qi group(P>0.05). (3) The apoptosis cells in replenishing qi or activating blood group were decreased significantly(P<0.01),and the effect of replenishing qi was better than that of activating blood(P<0.01). Conclusion:(1)Cell proliferation is decreased and apoptosis is increased in the liver tissue of natural aging rats. (2) Recipes replenishing qi or activating blood can accelerate cell proliferation in the liver tissue of natural aging rats,and the effect of activating blood was slightly stronger than that of replenishing qi. (3) Recipes replenishing qi or activating blood can inhibit cell apoptosis in the liver tissue of natural aging rats,and the effect of replenishing qi was better than that of activating blood.