益气泄浊法对慢性肾功能不全患者TNF-α及其sTNFR水平影响研究

The Effect of Tonify-qi and Remove-turbid Therapy on TNF-α and sTNFR of Patients with Chronic Renal Failure(CRF)

目的 :研究益气泄浊法对慢性肾功能不全患者 (CRF)血清肿瘤坏死因子 (TNF α)及其可溶性受体 (sTNFRⅠ、Ⅱ )含量的影响 ,进一步探讨益气泄浊法治疗慢性肾功能不全有效的作用机制。方法 :采用ELISA法检测 2 8例CRF患者服用益气泄浊中药前后血清TNF α及其sTNFRⅠ、Ⅱ含量 ,并与 2 2例服用尿毒清冲剂的CRF患者及 2 0名健康人作对照。结果 :(1 )CRF患者血清TNF α及其sTNFRⅠ、Ⅱ含量明显高于健康组 ,与肌酐 (SCr)、尿素氮 (BUN)、胆固醇 (TC)、甘油三脂 (TG)、尿蛋白定量、血色素(Hb)、血清白蛋白 (ALB)、高密度脂蛋白 (HDL)呈正相关。 (2 )两个疗程后两组CRF患者血清TNF α及其sTNFRⅠ、Ⅱ含量及其它各项指标均降低 ,但治疗组降低更明显 ,与对照组比较差异显著 (P <0 0 5)。结论 :CRF患者血清TNF α及其sTNFRⅠ、Ⅱ含量与病情程度密切相关 ,病情越重 ,其含量越高 ,益气泄浊中药有降低CRF患者血清中增高的TNF α及其sTNFRⅠ、Ⅱ含量的作用 ,并可改善其它各项相关指标 ,减轻CRF患者的肾功能损害 。

Objective: Study the effect of tonify qi and remove turbid(TQRT)therapy on serum tumor necrosin(TNF α)and its soluble receptor(sTNFRⅠ,Ⅱ) of patients with CRF, further explore the effective emchanism for the method to treat CRF. Method:Use ELISA method to test the contents of TNF α and sTNFRⅠ,Ⅱ before and after the 28 CRF patients taking TQRT drugs, and compare with that of the CRF patients taking Niaoduqing infusion and 20 normal healthy persons. Result:(1)The CRF patients' TNF α and sTNFRⅠ,Ⅱ were obviously higher than that of healthy group, appearing positively realted with Ser, BUN, TC, TG, urinary protein fixed quantity, Hb, ALB and HDL. (2)After 2 courses of treatment, the contents of TNF α and sTNFRⅠ,Ⅱ and other indexes of the 2 groups of CRF patients were decreased, while that of treatment group was much lower, with obvious difference compared with control group( P <0 05). Conclusion: For the CRF patients,the serum TNF α and sTNFRⅠ,Ⅱ were closely related with the disease development, more contents, more severe the disease was, TQRT drugs could reduce the risen contents of TNF α and sTNFRⅠ,Ⅱ in the serum of CRF patients, improve other concerned indexes, alleviate the CRF patients' kidney injury and protect the kidney function.