轮状病毒培养纯化及重组子代分析

CELL CULTURE AND GENOMIC ANALYSIS OF REASSORTANTS OF ROTAVIRUSES

人轮状病毒Wa和Hu7株细胞培养条件中,胰蛋白酶处理和旋转培养,在病毒增殖和细胞病变生成过程中起关键作用。通过对一株人轮状病毒FH4232株与猴轮状病毒SA11株的混合感染株的体外培养和空斑纯化,发现子代克隆中有2株FH4232与SA11株生成的重组子代。重组子代病毒株的第5、11条泳带来自亲代FH42321株,其余多条则来自亲代SA11株。体外轮状病毒重组子代的发现对研究其基因重组变异有重要意义,而且为疫苗制备和应用提供有效途径。

In propagation of human rotaviruses (HRV) and induction of typical cytopathic effects (CPE) of infected cells, trypsin treatment and roller culture technics were of crucial importance During cloning a virus mixture mixed by HRV FH4232 with Simian Rotavirus SA11, two reassortants of FH4232 and SAll were obtained. From analysis on viral RNA, it was found that the 5th and 11th segments of reassortant RNA electropherotype were stemed from parental RNA of strain FH4232, and the others from SA11. Rotavirus reassortants generated in vitro might account for the variability of HRV in vivo. In addition, these human-animal rotavirus reassortants would be offered to promise as potential vaccine candidates.