急性冠状动脉综合征患者循环中NF-κB活性的变化及临床意义

The change of levels of activated NF-κB in the blood of patients with acute coronary syndrome and its significance

目的 研究急性冠状动脉综合征 (ACS)患者循环中核因子κB(NF κB)活性的变化规律及临床意义。方法 采用酶联免疫吸附法 (ELISA)测定 76例入选患者周围血白细胞中NF κB的活性。分为对照、稳定型心绞痛 (SAP)、不稳定型心绞痛 (UAP)、急性心肌梗死 (AMI) 4组。结果 对照组NF κB活性为 ( 0 6 1± 0 35 ) μg ,稳定型心绞痛 (SAP)为 ( 0 5 9± 0 39) μg ,两组比较P >0 0 5。在 0～12h、12～ 2 4h、2 4～ 4 8h、1周时段UAP患者NF κB活性分别为 ( 1 12± 0 10 ) μg,( 1 4 1± 0 18) μg ,( 1 18± 0 13) μg ,( 0 82± 0 18) μg。AMI各时段NF κB的值分别为 ( 1 2 8± 0 14 ) μg,( 1 6 9± 0 4 1) μg ,( 1 5 5 ± 0 4 5 ) μg ,( 0 89± 0 0 6 ) μg。对照组和SAP组分别与UAP组、AMI组各时段比较差异均有显著性 ,P<0 0 1。结论 NF κB在ACS患者循环中活性增高 ,与ACS患者急性心肌缺血事件有密切的关系 ,可能反映冠心病病情的活动情况 ,并在UAP及AMI中有不同的演变规律。

Objective To investigate the change of levels of activated Nuclear Factor κB (NF κB) in the blood of patients with acute coronary syndrome (ACS) and its significance Methods Seventy six patients were divided into four groups: control group,stable angina pectoris group (SAP), unstable angina pectoris group (UAP), and acute myocardial infarction group (AMI) NF κB was measured with ELISA Results The level of activated NF κB was (0 61±0 35) μg in control group and (0 59±0 39) μg in SAP group, and (1 12±0 10) μg, (1 41±0 18) μg, (1 18±0 13) μg, (0 82±0 18) μg in UAP group and (1 28±0 14) μg, (1 69±0 41) μg, (1 55±0 45) μg, (0 89±0 06) μg in AMI group at 0～12 h, 12～24 h, 24～48 h, and 1 w time intervals respectively The levels of activated NF κB were higher in UAP and AMI groups than that in control group or SAP group ( P <0 01) and changed differently with time after chest pain in UAP and AMI groups Conclusion The levels of activated NF κB were markedly elevated in ACS patients and changed differently with time after episode of angina There is a close relationship between NF κB activation and acute myocardial ischemia in ACS patients Furthermore, NF κB activation may reflect coronary artery disease activity