骨形成蛋白一多孔羟基磷灰石复合人工骨的实验研究

Experimental Studies on the Bone Morphogenetic Protein/Hydroxyapatite Composite(BMP/HA)as a Bone Substitute

将牛骨形成蛋白(bBMP)与多孔羟基磷灰石(HA)结合制成 bBMP-HA 复合人工骨,植入狗股骨缺损区,植入后2,4,8,16,24周,采用组织学、扫描电子显微镜及^(43) Ca 同位素示踪综合考查其骨修复过程,并以单纯 HA 作为对照。结果发现,各实验程期 bBMP-HA 材料内新骨形成量均明显多于单纯 HA 对照组,bBMP-HA 植入后早期诱导骨形成较活跃,实验证明 bBMP-HA 复合人工骨具有良好的诱导成骨能力,并能促进骨缺损的修复。

Bone morphogenetic protein(BMP)was extracted from bovine bones.BMP/HA com-posite was prepared by combining BMP with porous HA cylinders with diameter of 4mmand length of 12～15mm.The BMP/HA composite contains 5mg bBMP,with a porous sizeof HA cylinder ranging from 100μm to 300μm.The BMP/HA composite and HA alone(experimental control)were implanted intothe bone defects of femur in 16 dogs.Comparative study was performed by using histolog- ical methods,scanning electron microscopy(SEM)and scintigraphy as ^(45)Ca-labelled tra-cer.Observation at 2,4,8,16,24 weeks postoperatively showed that not only werethe speed and time of new bone ingrowing into the BMP/HA composite much quickerand earlier than that of implants of HA alone(experimental control gruop),but also thequantity of new bone ingrowin into the BMP/HA composite was much higher than thatof HA alone implants.It is clear that the BMP/HA composite presented a very activeability of osteoinduction,and repaired the bone defect in a relatively short time,while theimplant of HA alone only plays a growth-guiding properties(osteoconduction)causing newbone to grow into the bonedefects in a relatively long time.SEM showed that there was aintimate bone-implant contact in all implants.The results of quantitive analysis of incor-poration of ^(45)Ca into new bone indicated the bone ingrowth of BMP/HA composite at high-er rate within 3 weeks.The results of this study suggested that BMP/HA composite poss-ess a superior ability of osteogenesis.