用铈作捕捉剂检测横纹肌细胞Ca^(2+)-ATP酶活性

CERIUM-BASED CYTOCHEMICAL LOCALIZATION OF Ca^(2+)-ATPase ACTIVITY IN RAT STRIATED MUSCLE CELL

本文介绍了一种新捕捉剂铈在横纹肌Ca^(2+)-ATP酶电镜细胞化学中的应用。与铅法相比,以铈为捕捉剂的电镜细胞化学方法有反应稳定、非特异性反应少、反应产物细而均匀等优点。将孵育液pH由铅法的8.8～9.0改为7.2～7.4,能显示肌膜Ca^(2+)-ATP酶的活性。

A new cytochemical method for Ca^(2+)-ATPase detection using cerium as a capture agent has been described in this paper. Cerium is a better capture agent for inorganic phosphate than lead. The cytochemical reaction is more stable and reproducible. Ultrastructurally, cerium phosphate reaction products are fine electron-dense precipitates. Furthermore, nonspecific reaction products commonly encountered in the lead-based phosphatase reactions are virtually nonexistent when cerium is used as the capture agent. Sarcolemmal Ca^(2+)-ATPase activity can be observed in cerium-based method (pH 7.4). but can not be seen in the lead-based method (pH8.8-9.0).