实时荧光定量PCR法检测淋病奈瑟菌感染的效果

REAL-TIME FLUORESCENCE QUANTITATIVE POLYMERASE CHAIN REACTION FOR DETECTION OF NEISSERIA GONORRHOEAE INFECTION

①目的 应用实时荧光定量聚合酶链反应 (FQ PCR)技术准确定量检测临床标本中淋病奈瑟菌基因 ,同时与金标准———细菌培养法比较其灵敏度和特异度 ,为临床淋病的诊疗提供依据。②方法 采集 173例病人临床标本 ,以PE5 70 0全自动PCR扩增分析仪和淋病奈瑟菌实时荧光定量PCR检测试剂盒进行核酸检测。同时 ,用含PolyViteX的巧克力板对淋病奈瑟菌进行分离 ,用ATBExpression自动细菌鉴定仪及配套的APINH进行鉴定。③结果 173份临床标本中 ,荧光定量PCR法示 4 7例淋病奈瑟菌阳性 ,阳性率为 2 7.2 % ;细菌培养法示 4 1例淋病奈瑟菌阳性 ,阳性率为 2 3.7%。与细菌培养法相比 ,荧光定量PCR法的灵敏度为 10 0 % ,特异度为 95 .5 % ,两者的符合率为 96 .5 %。④结论 荧光定量PCR技术具有简便、快捷、灵敏度高、特异度高、定量准确等优点 。

Objective To detect Neisseria gonorrhoeae DNA in genitourinary specimens by real-time fluorescence quantitative Polymerase Chain Reaction (FQ-PCR), and to provide a clinical basis for the diagnosis and treatment of gonorrhoea. Methods The study consisted 173 specimens, which were detected for DNA with Neisseria gonorrhoeae real-time FQ-PCR. The specimens were cultured as well. Results Of the 173 clinical specimens, real-time FQ-PCR showed that 47 were positive(27.2%);while 41 cases(23.7%) were positive by the culture method. Compared to the culture method, the specificity, sensitivity, and agreement of FQ-PCR was 95.5%, 100%, and 96.5%, respectively. Conclusion FQ-PCR is a simple, rapid, sensitive and specific method to detect Neisseria gonorrhoeae.