左旋吡喹酮对日本血吸虫皮层损害的扫描电镜观察

SCANNING ELECTRON MICROSCOPIC OBSERVATIONS ON TEGUMENTAL DAMAGE OF SCHISTOSOMA JAPONICUM AFTER TREATMENT WITH LEVO-PRAZIQUANTEL

感染血吸虫小鼠用左旋吡喹酮75mg/kg、吡喹酮150mg/kg灌胃给药,经不同时间收集成虫,作扫描电镜观察。虫体出现皮层肿胀、褶嵴融合、糜烂、剥脱及白细胞粘附等病变。结果提示,用药初期,葡萄糖的吸收障碍可能是影响虫体的主要因素。在后期,抗原暴露所致宿主免疫反应,可能在左旋吡喹酮杀虫机理中起主要作用。

Scanning electron microscope (SEM) observations were made of changes in the tegument of Schistosoma japonicum from mice after treatment with a single oral dose of praziquantel (150mg/kg) and levo-praziquantel (75mg/kg). Infected mice were sacrificed 10min, 30min, 1h, 4h, 12h, 24h, 48h, after administration of drugs. Worms were collected and prepared for SEM observation.Changes observed in the tegument of worms after administration of levo-praziquantel or praziquantel were the same. They included: Swelling, wrinkling and fusion of tegumental folds, enlargment of sensory structures, erosion and exfoliation of the tegumental surface, and attachment of host leucocytes to the denuded surfaces. The results suggested that the tegumental damage may disturb glycogen absorption of the worm; erosion and exfoliation of its tegumental surface with resulting exposure of worm antigen may activate immune response of the host.These are perhaps the two factors killing the worms during the early and late stages, respectively.