大肠癌癌周粘膜酸性粘蛋白组织化学观察

HISTOCHEMICAL CHANGES IN ACIDIC MUCINS OF THE MUCOSA SURROUNDING COLORECTAL CARCINOMA

以高铁双胺-奥蓝粘蛋白组织化学方法观察43例大肠癌癌周粘膜酸性粘蛋白组织化学变化,发现大肠癌癌周普遍存在移行粘膜,其细胞唾液酸性粘蛋白含量明显增加、硫酸粘蛋白含量减少或消失,其组织学改变常表现为腺管仲长、弯曲、扩张;上皮细胞数量增多、体积增大、核分裂相增多;不成熟上皮细胞数量增多。癌旁近、远侧移行粘膜范围相差不显著;移行粘膜范围与Dukes分期无显著相关性,提示发生细胞唾液酸粘蛋白组织化学改变的癌周移行上皮代谢旺盛、分泌活跃,系不稳定的癌前期改变。

Forty-three specimens of colorectal carcinoma were studied with HID-AB mucin histochemical stain to investigate the changes in the acidic mucin of the mucosa surrounding the tumor. Transitional mucosa was identified around the tumor. It had a considerably high content of sialomucin while the sulphomucin content was markedly decreased. Histologically, the transitional mucosa had elongated, dilated crypts and a lot of immature and intermediate cells. The transitional mucosa extended 3.63±3.31cm proximally and 4.02±3.32cm distally from the tumor. No obvious correlation was shown between the extent of transitional mucosa and Dukes' classification of the tumors. It was postulated that the transitional mucosa might be a primary unstable precancerous lesion.